南方医科大学学报

南方医科大学学报 ›› 2021, Vol. 41 ›› Issue (6): 931-936.doi: 10.12122/j.issn.1673-4254.2021.06.17

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LncRNA SNHG3对宫颈癌细胞SiHa增殖、迁移及侵袭的影响

孙子久，胡 静，胡 凯，唐 敏，孙恃雷，方玉婷，余伙梅，张 彦   

  • 出版日期:2021-06-20 发布日期:2021-07-05

Role of long noncoding RNA SNHG3 in regulating proliferation, migration and invasion of cervical cancer SiHa cells

  • Online:2021-06-20 Published:2021-07-05

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摘要： 目的 探究长链非编码RNA（lncRNA）小核仁RNA宿主基因3（SNHG3）对人宫颈癌细胞系SiHa增殖、迁移及侵袭的影响。方法 利用 GEO 数据库芯片分析 lncRNA SNHG3 在正常宫颈组织和宫颈癌样本中的表达；通过实时定量 PCR 检测LncRNA SNHG3、上皮-间质转化标志物 N-cadherin、Snail、Vimentin 和 E-cadherin 基因表达水平；通过 Western blot 检测 N-cadherin、Snail、Vimentin和E-cadherin蛋白表达水平；在SiHa细胞中转染siRNA干扰片段来敲低lncRNA SNHG3，并采用qRT-PCR验证转染效率；通过CCK8实验检测细胞增殖能力，划痕愈合实验检测细胞横向迁移能力，Transwell小室实验检测细胞纵向迁移及侵袭能力。结果 LncRNA SNHG3在宫颈癌组织及细胞系中高表达；敲低lncRNA SNHG3后，明显抑制SiHa细胞的增殖能力（P<0.001）、迁移能力（P<0.01）和侵袭能力（P<0.001）；qRT-PCR和Western blot结果显示敲低lncRNA SNHG3可下调N-cadherin、Snail和Vimentin表达水平（P<0.001），同时上调E-cadherin表达水平（P<0.001）。结论 LncRNA SNHG3可通过激活EMT通路促进宫颈癌细胞SiHa增殖、迁移及侵袭能力。

关键词: 宫颈癌；长链非编码RNA；SNHG3；增殖；迁移；侵袭

Abstract: Objective To investigate the regulatory role of the long non-coding RNA (lncRNA) small nucleolar host gene 3 (SNHG3) in proliferation, migration and invasion of human cervical cancer cell line SiHa. Methods Array data were retrieved from GEO database to analyze the expression levels of SNHG3 in cervical cancer and adjacent normal tissues. SiHa cells were transfected with a small interfering RNA (siRNA) targeting SNHG3, and the changes in the transcriptional levels of lncRNA SNHG3 and the epithelial-mesenchymal transition (EMT) markers N-cadherin, Snail, vimentin and E-cadherin were detected using real-time quantitative PCR; the protein expressions of N-cadherin, Snail, vimentin and E-cadherin were determined using Western blotting. Cell counting kit-8 (CCK8) assay was utilized to assess the proliferation capacity of the transfected cells. Wound healing assay and Transwell assay were performed to evaluate the transversal and longitudinal migration and invasion abilities of the cells. Results SNHG3 was over-expressed in cervical cancer tissues and SiHa cells. In SiHa cells, knocking down SNHG3 significantly inhibited the proliferation (P<0.001), migration (P<0.01) and invasion abilities (P<0.001) of the cells, down-regulated the expression levels of N-cadherin, Snail and vimentin (P<0.001) and up-regulated the expression of E-cadherin (P<0.001). Conclusion SNHG3 may promote the proliferation, migration and invasion of SiHa cells by activating the EMT signaling pathway.

Key words: cervical cancer; long non-coding RNA; small nucleolar host gene 3; proliferation; migration; invasion