南方医科大学学报

南方医科大学学报 ›› 2021, Vol. 41 ›› Issue (6): 909-915.doi: 10.12122/j.issn.1673-4254.2021.06.14

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Mn2+掺杂普鲁士蓝纳米颗粒T1-T2双模磁共振成像和光热治疗的体外研究

何庚翰，陶 泉，刘 闯，张 迪，周宇平，刘瑞源   

  • 出版日期:2021-06-20 发布日期:2021-07-05

Mn2+ doped Prussian blue nanoparticles for T1-T2 dual-mode magnetic resonance imaging and photothermal therapy in vitro

  • Online:2021-06-20 Published:2021-07-05

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摘要： 目的 制备一种Mn2+掺杂普鲁士蓝纳米颗粒，用于检测体外T1-T2双模磁共振成像和光热治疗效果。方法 采用微乳液法，以二氯化锰、氯化亚铁和铁氰化钾为原料制备Mn2+掺杂普鲁士蓝纳米颗粒；观察纳米颗粒的体外T1-T2双模磁共振成像性能；考察808 nm 激光照射后纳米颗粒的升温效果；通过CCK-8法和AM/PI活死细胞染色法观察体外光热治疗效果。结果 成功制备了Mn2+掺杂普鲁士蓝纳米颗粒，粒径为39.46±0.42 nm，电位为-25.9±1.2 mV，粒径均匀，分散性好；808 nm激光照射10 min 后，纳米颗粒分散系可以升温至 90 ℃。由 7T 磁共振测得的数据拟合获得 r1 值为 0.68（mmol/L）-1s-1，r2 值为 3.65（mmol/L）-1s-1，具有良好的T1-T2双模磁共振成像效果；在808 nm激光照射下，可显著降低细胞存活率（P<0.05）；CCK-8 法和AM/PI活死细胞染色法的结果均显示纳米颗粒对HepG2细胞具有良好的光热治疗效果。结论 成功制备了粒径均一的Mn2+掺杂普鲁士蓝纳米颗粒，该纳米颗粒的T1-T2双模磁共振成像效果和体外光热治疗效果良好，且无明显细胞毒性。

关键词: 普鲁士蓝；微乳液；锰掺杂；磁共振成像；光热治疗

Abstract: Objective To prepare Mn2+-doped Prussian blue nanoparticles (Mn-PB NPs) for T1-T2 dual-mode magnetic resonance imaging (MRI) and photothermal therapy in vitro. Methods Mn-PB NPs were prepared based on manganese chloride, ferrous chloride and potassium ferricyanide using the microemulsion method. The performance of T1-T2 dual-mode MRI with Mn-PB NPs and the photothermal property of the nanoparticles were assessed. CCK-8 assay and AM/PI double staining were used to evaluate the effect of photothermal therapy in vitro using the parepared nanoparticles. Results The prepared Mn-PB NPs had a mean particle size of 39.46±0.42 nm with a Zeta potential of -25.9±1.2 mV and exhibited a good dispersibility and uniform particle size. In MRI using the nanoparticles, the r1 and r2 values reached 0.68 and 3.65 (mmol/L)-1s-1 , respectively, indicating good performance of Mn-PB NPs for T1 and T2 enhancement in MRI. When irradiated with 808 nm laser for 10 min, Mn-PB NPs showed a temperature rise to 90 ℃ to cause significant reduction of cell survival. CCK-8 assay and AM/PI double staining confirmed that Mn-PB NPs were capable of efficient killing of HepG2 cells upon 808 nm laser irradiation. Conclusion The Mn-PB NPs prepared in this work have uniform particle size and show good performances both in MRI for T1 and T2 enhancement and in photothermal therapy in vitro without obvious cytotoxicity.

Key words: Prussian blue; microemulsion; Mn doping; magnetic resonance imaging; photothermal therapy