南方医科大学学报

南方医科大学学报 ›› 2020, Vol. 40 ›› Issue (10): 1406-1414.doi: 10.12122/j.issn.1673-4254.2020.10.05

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高脂饮食和运动影响妊娠中期大鼠皮下和腹膜脂肪组织中asprosin和CTRP6的表达

杨 昭，蒋佳男，黄佳琪，招雨佳，罗 肖，宋 琳   

  • 出版日期:2020-10-20 发布日期:2020-10-20

Effect of high-fat diet and exercise on asprosin and CTRP6 expression in subcutaneous and retroperitoneal adipose tissues in rats during mid-gestation

  • Online:2020-10-20 Published:2020-10-20

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摘要： 目的 研究孕期高脂饮食和运动对皮下和腹膜后脂肪组织中asprosin和CTRP6表达的影响。方法 从妊娠第1天开始，大鼠分别以脂肪含量13.5%的常规饲料和脂肪含量60%的高脂饲料喂养，妊娠第3天运动组大鼠放开转轮开始自主运动，饮食和运动处理持续整个孕期。按照处理将妊娠Sprague-Dawley大鼠随机分为4组：正常饮食非运动组（CH-SD）、正常饮食运动组（CH-RW）、高脂饮食非运动组（HF-SD）、高脂饮食运动组（HF-RW）。于妊娠第14天用RT-qPCR测定脂肪组织中asprosin和CTRP6 的 mRNA 表达水平，Western blotting 测定 asprosin 和 CTRP6 的蛋白表达水平。结果 腹膜后脂肪组织中，asprosin mRNA表达在HF-RW组显著高于CH-SD组、CH-RW组和HF-SD组（P<0.001）。皮下脂肪组织中，asprosin mRNA表达在HFSD 组显著高于 CH-SD 组（P=0.0234），而 HF-RW 组中 asprosin mRNA的表达水平正常（P=0.0494）。腹膜后脂肪组织中，CTRP6 mRNA水平在HF-RW组显著高于CH-SD组、CH-RW组和HF-SD组（P<0.0001），CTRP6蛋白水平在HF-RW组显著高于 CH-RW组和 HF-SD 组（P<0.05）。皮下脂肪组织中，CTRP6 蛋白表达在 CH-RW组显著高于 HF-SD 组和 HF-RW组（P<0.05）。皮下脂肪组织中CTRP6的蛋白水平与血糖呈显著负相关（r=-0.6038，P=0.0172），而腹膜后脂肪组织中CTRP6的蛋白水平与血糖呈显著正相关（r=0.5305，P=0.0285），且asprosin和CTRP6 mRNA在皮下脂肪组织中的表达量要显著低于其在腹膜后脂肪组织中的表达量（P<0.0001）。结论 妊娠期高脂饮食和运动会影响妊娠中期大鼠脂肪组织内asprosin和CTRP6的表达水平，且具有部位差异性。

关键词: asprosin, CTRP6, 高脂饮食, 孕期运动, 脂肪组织

Abstract: Objective To study the effects of high-fat (HF) diet and exercise on the expressions of asprosin and CTRP6 in adipose tissues in different regions of rats during mid-gestation. Methods Pregnant SD rats were fed on a standard chow diet or a high-fat (60% fat content) diet for 14 days starting on gestation day (GD) 1. Starting from GD3, the rats fed either on normal or high-fat diet in the exercise groups (CH-RW and HF-RW groups) were allowed access to the running wheels for voluntary running, and those in sedentary groups (CH-SD and HF-SD groups) remained sedentary. At the end of the 14 days, adipose tissues were sampled from different regions of the rats for detecting the mRNA and protein expressions of asprosin and CTRP6 using RT-qPCR and Western blotting. Results The mRNA expression of asprosin in retroperitoneal adipose tissues was significantly higher in HF-RW group than in the other 3 groups (P<0.0001). Asprosin mRNA expression in subcutaneous adipose tissues was significantly higher in HF-SD group than in CH-SD group (P=0.0234) and comparable between HF-RW and CH-SD groups (P=0.0494). CTRP6 mRNA expression in retroperitoneal adipose tissues was also significantly higher in HF-RW group than in the other groups (P<0.0001), and CTRP6 protein expression was signficiantly higher in HF-RW group than in CH-RW and HF-SD groups (P<0.05). In subcutaneous adipose tissues, CTRP6 mRNA expression was significantly higher in CH-RW group than in HF-SD and HF-RW groups (P<0.05). The protein expression level of CTRP6 in subcutaneous adipose tissues showed a significant negative correlation with blood glucose (r=-0.6038, P=0.0172), while its expression in retroperitoneal adipose tissues was positively correlated with blood glucose (r=0.5305, P=0.0285); the mRNA expression levels of asprosin and CTRP6 were significantly lower in subcutaneous than in
retroperitoneal adipose tissues (P<0.0001). Conclusion High-fat diet and exercise during mid-gedtation can affect the expression levels of asprosin and CTRP6 in adiposetissues of rats in a site-specific manner.

Key words: asprosin, CTRP6, high-fat diet, exercise during pregnancy, adipose tissue