南方医科大学学报

南方医科大学学报 ›› 2020, Vol. 40 ›› Issue (02): 233-239.doi: 10.12122/j.issn.1673-4254.2020.02.10

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氢气水通过升高Nrf2的表达减轻百草枯诱导的肺纤维化

李 婷，邓树豪，雷 雯，李振坤，吴文娟，张 涛，董昭兴   

  • 出版日期:2020-03-14 发布日期:2020-02-20
  • 基金资助:

Hydrogen water alleviates paraquat-induced lung fibroblast injury in vitro by enhancing Nrf2 expression

  

  • Online:2020-03-14 Published:2020-02-20

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摘要： 目的 探讨氢气水依赖Nrf2对百草枯诱导的肺成纤维细胞增殖，转化及胶原分泌的影响。方法 人肺成纤维细胞（HFL-1）体外培养，采用RNA干扰技术沉默HLC-1细胞中Nrf2的表达，设置对照组、百草枯（PQ）染毒组、PQ+氢气水治疗组、PQ+氢气水+Nrf2siRNA综合干预组。设置对照组：人肺成纤维细胞（HFL1）正常培养；染毒组：HFL1+600 μmol/L PQ 24 h；氢气水治疗组：HFL1+600 μmol/L PQ24 h后+氢气水；综合干预治疗组：HFL1转染siRNA+600 μmol/LPQ 24 h后+氢气水。MTT法检测细胞的增殖，Western blot、qt-PCR、免疫荧光检测各组肺成纤维细胞Col-Ⅰ、Col-Ⅲ、α-SMA、Nrf2的表达，同时ELISA检测抗氧化物SOD、CAT、GSH的含量。结果 氢气水治疗组较PQ染毒组Col-Ⅰ、Col-Ⅲ、α-SMA的表达明显降低，干扰Nrf2基因表达后，氢气水疗效较治疗组显著下降（P<0.05）。氢气水治疗组Nrf2表达量较PQ染毒组增加（P<0.05）。结论 氢气水可能通过诱导Nrf2的表达促进肺成纤维细胞的增殖和抗氧化物的生成，同时抑制其转化和分泌功能。

Abstract: Objective To investigate the effects of hydrogen water on proliferation, differentiation, collagen secretion and Nrf2 expression in paraquat-induced human lung fibroblasts. Methods In vitro cultured human lung fibroblasts (HFL1) exposed to 600 μmol/L paraquat (PQ) for 24 h were treated with hydrogen water with or without RNA interference of Nrf2 expression. The changes in the cell proliferation were examined using MTT assay, and the expressions of Col-I, Col-III, α-SMA and Nrf2 in the cells were detected using Western blotting, real-time quantitative PCR and immunofluorescence assay. The contents of SOD, CAT and GSH in the cells were determined with ELISA. Results Compared with the PQ-exposed cells, the cells with hydrogen water treatment showed significantly lowered expressions of Col-I, Col-III, and α-SMA. Interference of Nrf2 expression obviously attenuated the effect of hydrogen water on PQ-exposed cells. Hydrogen water treatment significantly increased the expression of Nrf2 and promoted the production of the antioxidants in PQ-exposed lung fibroblasts. Conclusion Hydrogen water enhances Nrf2 expression to promote the proliferation and production of antioxidants and inhibit the differentiation and collagen secretion in PQ-exposed human lung fibroblasts in vitro.