南方医科大学学报

南方医科大学学报 ›› 2019, Vol. 39 ›› Issue (10): 1253-.doi: 10.12122/j.issn.1673-4254.2019.10.19

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胎盘植入组织lncRNA表达特征与ceRNA调控网络构建

吴淑贞，张慧珊，刘雁，王瑞，叶少欣，曾萌，刘正平   

  • 出版日期:2019-10-20 发布日期:2019-10-20

Long non-coding RNAs show different expression profiles and display competing endogenous RNA potential in placenta accreta spectrum disorders

  • Online:2019-10-20 Published:2019-10-20

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摘要： 目的探讨lncRNA在胎盘植入中的表达特征及潜在的ceRNA调控机制。方法随机选取2017年12月~2018年6月于我 院行剖宫产合并胎盘植入的植入部分胎盘组织与邻近正常胎盘组织各5例，采用基因芯片技术检测组织lncRNA的表达水平； 植入组与对照组比较，筛选差异表达lncRNAs；随机选取5个差异表达lncRNAs进行实时荧光定量PCR检测，验证基因芯片结 果的准确性与可靠性，对差异表达基因进行GO功能聚类分析及KEGG 通路分析；选取芯片扫描与qRT-PCR结果均显示显著差 异的ENST00000511361（RP5-875H18.4）、NR_027457（LINC00221）和NR_126415（FOXP4-AS1）3条lncRNAs构建ceRNA调 控网络。结果植入组与对照组比较，筛选出329个差异表达lncRNAs与179个差异表达mRNAs，实时荧光定量PCR变化趋势 与芯片结果相一致；差异表达mRNAs主要参与TGF-β通路的调控；ceRNA调控网络的构建提示RP5-875H18.4--miRNA-218-- SLIT2在胎盘植入的发生中存在潜在ceRNA调节机制。结论差异表达lncRNAs可能通过调控TGF-β通路参与胎盘植入的发 生发展过程，RP5-875H18.4--miRNA-218--SLIT2在胎盘植入的发生中存在潜在ceRNA调节机制，但该结果还需进一步验证。

Abstract: Objective To investigate the expression profile of long non-coding RNAs (lncRNA) and identify potential lncRNA-related competing endogenous RNAs (ceRNA) in placenta accrete spectrum disorders (PAS). Methods Five tissue specimens of placental implantation and 5 adjacent normal placental tissues were collected from cesarean section deliveries complicated by PAS in our hospital between December, 2017 and June, 2018. Human microarrays were used to identify the lncRNAs that were differentially expressed in PAS, and 5 of the identified lncRNAs were further validated using qRT-PCR. GO and KEGG pathway analyses were performed to indentify the most significant enrichment functions. A ceRNA network was constructed based on ENST00000511361 (RP5-875H18.4), NR_027457 (LINC00221) and NR_126415 (FOXP4-AS1) to pinpoint the potential lncRNAs-related ceRNA. Results A total of 329 lncRNAs and 179 mRNAs were identified to have differential expression in PAS. The results of qRT-PCR were consistent with the human microarrays results. Transforming growth factor-β (TGF-β) signaling pathway was the most significantly enriched pathway. The constructed ceRNA network suggested that RP5-875H18.4--miRNA-218--SLIT2 had a potential ceRNA regulatory mechanism in PAS. Conclusion The differentially expressed lncRNAs are involved in the occurrence and progression of PAS possibly by regulating the TGF-β signaling pathway. The ceRNA network of RP5-875H18.4--miRNA-218--SLIT2 may play a role in the occurrence of PAS.