Multiple organ injury at early stage of intestinal and hepatic ischemia-reperfusion in rats

Abstract

Abstract: Objective To investigate the early-stage multiple organ injuries in rats subjected to intestinal and hepatic ischemia-reperfusion. Methods Seventy-five normal male Wistar rats were randomized equally into hepatic ischemia, intestinal ischemia and intestinal-hepatic ischemia groups. Before and at the end of occlusion (45 min), as well as at the time points of 0.5, 2.0 and 6.0 h during the reperfusion, respectively, 5 rats from each group were killed to obtain samples for determination of the contents of malondialdehyde (MDA), superoxide dismutase (SOD) in the blood, lung, kidney, pancreas and heart tissues, along with blood urea nitrogen (BUN), amylase (AMY), and creatine kinase MB (CK-MB). Results The activity of SOD was decreased (P<0.05) and MDA, BUN, AMY and CK-MB levels increased significantly (P<0.05) after ischemia-reperfusion as compared with those before ischemia. Conclusions Intestinal and hepatic ischemia-reperfusion can induce injury of multiple organs at early stage. With the same duration of ischemia-reperfusion, intestinal ischemia may induce severer injury than hepatic ischemia.

CLC Number:

R341.31

YANG Jin-cheng¹, WANG Zhi-wei², LI Chao-long¹, LIN Jian-hua¹, LIU Xing-guo¹, JI Qi-xing¹. Multiple organ injury at early stage of intestinal and hepatic ischemia-reperfusion in rats[J]. Journal of Southern Medical University, 2004, 24(02): 198-200,203.

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