Abstract: Objective The aim of this study was to investigate the protective effect of total saponins of Drukophora ginseng against CCl4- induced acute liver injury and the potential pharmacological mechanisms. Methods SD rats were randomly divided into normal group, model group, biphenyl bisabololol group (100 mg/kg), and total saponin of Druchysanthes chinensis low, medium, and high (50, 100, and 200 mg/kg) dosage group, with 8 rats in each group. Except for the blank group, the other groups used the CCl4-induced ALI model in rats, and the treatment group was given the intervention of positive liver formula by gavage in the modeling. The AST, ALT, TBil and alkaline phosphatase ALP levels of rats in each group were compared; HE was used to observe the histopathological changes in liver tissue; IHC was used to detect the expression of molecules related to PI3K/Akt/NF-κB signaling pathway in liver tissue; ELISA was used to determine the levels of T- SOD, GSH-Px, and MDA; WB was used to detect the levels of PI3K-Akt and SIRT6-NF-κB pathway in liver tissue; and WB was used to detect the levels of PI3K-Akt and SIRT6-NF-κB pathway in liver tissue. -NF-κB pathway related protein expression in liver tissue. Results The network pharmacological analysis showed that total saponin of Druksen had therapeutic effects on ALI, and the key pathways were PI3K/Akt and other signaling pathways. The experimental results showed that serum AST, ALT, ALP, TBil and MDA were significantly increased in the model group (P<0.01), and the levels of T- SOD and GSH- Px were significantly decreased (P<0.01); the levels of ALT, AST, ALP, TBil and MDA were significantly decreased in the treatment groups (P<0.01), and the levels of T-SOD and GSH-Px were significantly increased (P<0.01). elevated (P<0.01).IHC showed that no positive expression of p-NF-κB was seen in the liver tissue cells of rats in the normal group, while the positive expression of p- NF- κB was significantly increased in the model group, and the number of positive cells was increased (P<0.01); the positive expression was significantly reduced in the treatment groups (P<0.01).WB showed that the expression of PI3K, p-Akt and SIRT6 proteins was decreased in the model group, and the level of p-NF- κB was significantly decreased in the model group, and the level of p- NF- Px was significantly decreased in the model group (P<0.01). levels decreased, and the expression levels of p-NF-κB, TNF-α and IL-6 proteins increased (P<0.05); the expression levels of PI3K, p- Akt and SIRT6 proteins in each treatment group significantly increased, and the expression levels of p- NF- κB p65, TNF- α and IL- 6 proteins significantly decreased (P<0.05). Conclusion Total saponins of bamboo ginseng can effectively slow down CCl4-induced acute liver injury in rats by regulating the PI3K/Akt and SIRT6/NF- κB pathways, exerting their anti- inflammatory, anti- oxidative stress and protective effects ALI.

Key words: Acute liver injury, Total saponins of panax japonicus, PI3K/Akt/NF-κB, anti-inflammation , anti-oxidation