Abstract: Objective To investigate the effect of tumor necrosis factor-α (TNF-α) on the release of matrix metalloproteinase-3
(MMP-3), MMP-9, and interleukin-17 (IL-17) in cultured mouse bone marrow-derived mast cells (BMMCs) in vitro. Methods
Primarily cultured mouse BMMCs at 8 weeks were exposed PBS (control) or TNF-α at the concentrations of 2, 10, or 50 ng/mL
for 12 or 24 h. Real-time PCR was performed to detect the mRNA expressions of MMP-3, MMP-9, and IL-17 in the exposed
cells. Results A 12-hour exposure of the BMMCs to TNF-α caused significantly increased expressions of MMP-3, MMP-9, and
IL-17 in a concentration-dependent manner (P<0.05). Prolonged exposures of the cells to 2 and 10 TNF-α for 24 h further
increased MMP-3, MMP-9, and IL-17 mRNA expressions, but exposure to 50 ng/mL TNF-α for 24 h increased only MMP-3 and
MMP-9 expressions but not IL-17 mRNA expression. Conclusions TNF-α treatment of primarily cultured BMMCs can
significantly increase the cellular expressions of MMP-3, MMP-9, and IL-17 mRNAin a time- and dose-dependent manner.