Abstract: Objective To explore the effects of methylglyoxal on endothelia cell migration. Methods Human umbilical vein
endothelial cells (HUVECs) were stimulated by serial concentrations of methylglyoxal (MGO, 0, 25, 50, 100 and 200 μmol/L)
for 24 h, and the cell migration was assessed by scratch wound and Transwell assay. The expression of integrin β3 in the
treated cells was examined by immunoblotting, and the effect of an anti-β3 antibody, LM609, on cell migration was
investigated. Results Methylglyoxal significantly inhibited HUVEC migration in a concentration-dependent manner (P<0.05).
Methylglyoxal decreased the expression of integrin β3 in a time- and concentration-dependent manner (P<0.05). LM609 also
significantly inhibited HUVEC migration (P<0.05). Conclusion Methylglyoxal inhibits HUVEC migration in vitro by
down-regulating integrin β3 expression.