Abstract: Objective To construct a recombinant lentivirus vector for Wilm’s tumor on X chromosome (WTX) gene and
establish a colorectal cancer SW620 cell line with stable WTX over-expression. Methods The full length coding region of WTX
gene was amplified with PCR, and the amplified fragment was cloned into the lentivirus vector GV387. The recombinant
lentivirus vector was transfected in 293T cells for packaging the virus, which was then transfected into colorectal cancer SW620
cells. The stably transfected cells were selected with G418, and the cellular expressions of WTX mRNA and protein were
detected using quantitative PCR and Western blotting. Results The recombinant plasmid was successfully constructed as
verified by sequence analysis. Quantitative PCR and Western blotting results showed that trasnfection with the recombinant
lentivirus significantly increased the expression levels of WTX in SW620 cells. Conclusion We successfully established a
colorectal cancer cell lines with stable over-expression of WTX, which provides an essential cell model for studying the role of
WTX in the tumorigenesis and progression of colorectal cancer.