Journal of Southern Medical University ›› 2015, Vol. 35 ›› Issue (06): 857-.
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Abstract: Objective To investigate the mitochondrial injury in enterovirus 71 (EV71)-infected Vero cells and explore thepossible mechanism. Methods A clinical isolate of EV71 was inoculated to Vero cells and the EV71 antigen was detected byimmunofluorescence assay. The morphological changes of Vero cells were observed using optical microscopy and transmissionelectron microscopy. The diameter and area density of the viral particles and the ratio and area density of vacuolatedmitochondria in the cells were measured on the ultrastructural images. Results EV71-infected Vero cells underwent obviouschanges and to a spherical morphology followed by cell death EV71 particles were detected in the cytoplasm byimmunofluorescence. Ultrastructurally, the infected cells contained a large number of viral particles in the cytoplasm, with aclustered distribution and lattice-like arrangement. The diameter of the particles were 16.3 nm and the mean area density was38.3% . Most of the mitochondria presented with swelling, vacuoles and degeneration. The ratio of the vacuolatedmitochondria was 90.9% with a mean area density of 89.2%. Viral particles were also found in some mitochondria. ConclusionEV71 proliferates in the cytoplasm and invades the mitochondria of infected Vero cells leading to mitochondrial injury and celldeath, suggesting that mitochondria are the targets for EV71 infection.
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URL: https://www.j-smu.com/EN/
https://www.j-smu.com/EN/Y2015/V35/I06/857