Abstract: Objective To breed neual stem cell-specific peroxisome proliferator-activated receptor γ (PPARγ) knockout mice.
Methods Two transgenic mouse models, namely B6.PPARγloxp/loxp and B6.Nestin-Cre were interbred, and the firstgeneration
offsprings were backcrossed with B6.PPARγloxp/loxp to obtain the second-generation mice. Genomic DNA was
extracted from the second-generation mice for PCR to amplify the loxp and Cre gene fragments followed by agarose gel
electrophoresis to verify their sizes. The mice with the PPARγloxp/loxp.Nestin-Cre (KO) genotype were selected as the neural
stem cell-specific knockout PPARγ mice, with B6.PPARγloxp/loxp (loxp) mice as the control. Tissue samples were collected
from specific regions of the mouse brain and peripheral tissue for detecting the expression of PPARγ mRNA using RT-PCR
and real-time quantitative PCR. Results and Conclusion Genotyping results showed PPARγloxp and Cre bands in the
knockout mice, which showed obviously decreased mRNA expression of PPARγ, suggesting successful establishment of
neural stem cell-specific PPARγ knockout mice. The two transgenic mice we used were fertile, and their breeding pattern
followed the laws of Mendelian inheritance.