Abstract: Objective To investigate the effect of miR-92b on the migration, adhesion and invasion of gastric cancer cell line
SGC7901. Methods The miR-92b inhibitor and mimics were transiently transfected in SGC7901 cells. The changes in the
migration, adhesion and invasion of the transfected cells were tested with wound healing assay, Transwell migration assay,
matrigel adhesion and Transwell invasion assay. The cellular expression of E-cadherin, vimentin, Akt and p-Akt were analyzed
by Western blotting. Results The migration, adhesion and invasion assays showed that transfection with the inhibitor of
miR-92b obviously decreased the numbers of gastric cancer cells. The expression of E-cadherin, AKT, and pAKT increased and
vimentin decreased significantly in the cells transfected with the inhibitor of miR-92b. Transfection with the mimics of miR-92b
produced opposite effects in SGC7901 cells. Conclusion miR-92b promotes the migration, adhesion and invasion of human
gastric cancer cell line SGC7901 by mediating epithelial-mesenchymal transition, and may accelerate tumor cell metastasis via
signaling pathways other than PI3K/Akt pathway.