Abstract: Objective To screen the down-stream proteins of transcription factor Sox2 and explore the role of Sox2 in the
proliferation and migration of colonic cancer cells in vitro. Methods The cellular proteins were separated by SDS-PAGE
electrophoresis and stained with Coomassie blue and amine plated silver. The differentially expressed proteins was identified
by mass spectrometry and verified by QPCR and Western blotting. A cell counting kit-8 (CCK8) assay was performed to
evaluate the cell proliferation, and the cell migration was assessed using Transwell assay. Results S3a was identified by
proteomics technology as a Sox2-downregulated protein while ENO1 and gama-actin the up-regulated proteins. QPCR and
Western blotting analyses showed that overexpression of Sox2 significantly decreased the expression of S3a (P<0.005) and
increased the expression of ENO1(P<0.05), but had no significant effect on gama-actin expression. Sox2 overexpression
obviously promoted cell proliferation and migration (P<0.05), while inhibition of Sox2 produced contrary effects (P<0.05).
Conclusion Sox2 negatively regulates S3a expression and positively regulates ENO1 expression to promot the proliferation
and migration of colonic cancer cells.