Abstract: Objective To investigate the cytotoxicity of normal CD8 + T lymphocytes retrovirally transduced with WT1
peptide-specific T-cell receptor (TCR) genes against human lung cancer cells. Methods HLA-A*2402-restricted and WT1
peptide-specific TCR-α/β genes were cloned from a cytotoxic T lymphocyte clone and inserted into a retroviral TCR expression
vector. The cytotoxicity of normal peripheral CD8 + T cells transduced with the WT1-TCR genes against human lung cancer
cells was evaluated using a standard 51Cr release assay. Results The WT1-TCR gene-modified T cells recognized the
peptide-pulsed target cells but not the non-pulsed cells. TCR-redirected CD8+ T cells lysed WT1-overexpressing human lung
cancer cells in an HLA-A*2402-restricted manner, but did not kill normal cells positively expressing HLA-A*2402. Conclusion
These data demonstrate the feasibility of adoptive immunotherapy with TCR-redirected T cell for the treatment of lung cancer.