Abstract: Objective To evaluate the effects of casein osphopeptide-amorphous calcium phosphate (CPP-ACP) in preventing
enamel demineralization. Methods Enamel blocks were prepared from premolars extracted from orthodontic patients. The
specimens were treated for 30 min daily for 7 days with one of the following agents: deionized water (negative control),
CPP-ACP paste, and NaF solutions (positive controls) (0.2% and 2%). After the treatments, the specimens were immersed in a
demineralizing solution (pH 4.5) for 7 days. The morphology and depth of the lesion were observed using scanning electron
microscopy, and the content of Ca, P, Mg in the demineralized enamel was measured by electron probe micro-analyzer.
Results The demineralization rates in all the treatment groups were significantly smaller than those in the control group after 7
days of demineralization. Conclusion The application of CPP-ACP to the enamel surfaces can inhibit enamel demineralization
with an equivalent effect to 0.2% NaF.