Journal of Southern Medical University ›› 2014, Vol. 34 ›› Issue (02): 188-.
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Abstract: Objective To investigate the changes in aorta morphology and Ca2 +-activated K+ (KCa) channel expression in thediabetic rats. Methods A diabetic rat model was established by a single intraperitoneal injection of streptozotocin (30 mg/kg)after a modified high fat and glucose diet for 8 weeks. Pathological changes in the aorta were observed with HE staining,elastic fiber staining, Masson’s trichrome staining and immunohistochemistry. Both the mRNA and protein levels of KCachannels in the aorta were measured by RT-PCR and Western blotting. Results Early atherosclerotic changes were observed inthe aorta wall of the diabetic rats. The mRNA and protein levels of KCa1.1 channel α- and β-subunits were significantlydecreased, while the expression of KCa3.1 channels was obviously enhanced in the middle layer of the aorta in the diabetic rats.Conclusion KCa channel switching in smooth muscles may play a role in the development of atherosclerosis in diabetic rats.
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https://www.j-smu.com/EN/Y2014/V34/I02/188