Journal of Southern Medical University ›› 2014, Vol. 34 ›› Issue (01): 92-.
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Abstract: Objective To study the effect of adenosine preconditioning on cell apoptosis and expressions of glucose-regulatedprotein (GRP-78) and cysteinylaspartate-specific protease 12 (caspase-12) in rats with spinal cord ischemia-reperfusion injury.Methods Twenty-seven rats were randomized into 3 equal groups and subjected to sham operation (group A), spinal cordischemia-reperfusion injury (group B), or ischemia-reperfusion injury with adenosine treatment. Spinal cordischemia-reperfusion injury was induced by cross-clamping of the abdominal aorta inferior to the left renal artery. The spinalcord function was assessed using the Modified Tarlov Scale at 6, 12, and 24 h after reperfusion. At 24 h after reperfusion,histological analysis was carried out with HE staining; cell apoptosis and viability were determined with TUNEL staining, andthe expressions of GRP-78 and caspase-12 proteins were determined with Western blotting. Results HE staining of the spinalcord showed extensive spinal cord injury such as cell edema in group B as compared with group C. Compared with group A,group B showed a significantly increased number of apoptotic cells; the number of apoptotic cells in group B was greater thanthat in group C. Compared with group B, group C showed significantly increased GRP-78 expression (P<0.01) and decreasedcaspase-12 expression (P<0.01). Conclusion Adenosine can up-regulate GRP-78 expression and down-regulate caspase-12expression, and protects the spinal cord against ischemia-reperfusion injury by inhibiting cell apoptosis.
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https://www.j-smu.com/EN/Y2014/V34/I01/92