Abstract: Objective To explore the appropriate acoustic parameters for augmenting the permeability of tumor cells using
microbubble-enhanced ultrasound. Methods Ninety intramuscular VX2 tumors grown in New Zealand white rabbits were
randomly divided into 4 parts by 4 factors, namely sound intensity, pulse width, insonation time and microbubble dose, each
at different levels. Therapeutic ultrasound was delivered directly to the tumor surface during intravenous infusion of
microbubbles. The tumor was excised after the treatment and sliced to investigate the change in tumor cell permeability using
lanthanum nitrate electron microscopy. The appropriate acoustic parameters of the 4 factors were investigated. Results
Sonoporation induced by microbubble-enhanced ultrasound augmented the permeability of the tumor cells, and the appropriate
sound intensity, pulse width, insonation time and microbubble dose were 0.43 W/cm2, 2.42 ms, 10 min, and 0.50 ml/kg,
respectively. Conclusion Sonoporation induced by microbubble-enhanced ultrasound can induce pores in tumor cell
membrane, which may potential increase the efficacy of tumor chemotherapy.