Abstract: Objective To identify the proteins involved in osteogenic differentiation of beagle dog bone marrow mesenchymal
stem cells (BMSCs) and explore its possible regulation mechanism of osteogenic differentiation of BMSCs. Methods Cultured
beagle dog BMSCs were induced by recombinant human bone morphogenetic protein-2 (rhBMP-2) for 7 days. The
differentially expressed proteins between cells with osteogenic differentiation and control cells were identified by proteomics
analysis based on two-dimensional gel electrophoresis. Q-PCR and Western blotting were used to verify the interested protein
of LASP1, ferritin light chain and heavy chain. Results Osteogenic differentiation was induced successfully in the BMSCs.
Twenty differentially expressed proteins were identified by proteomic analysis, including 9 down-regulated and 11
up-regulated ones. Q-PCR and Western blotting demonstrated a significant reduction of LASP1 expression and significant
up-regulation of ferritin in the BMSCs after a 7-day induction with rhBMP-2. Conclusion LASP1, which plays an important
role in the regulation of the activity of the cytoskeleton, and ferritin, an important molecule in cellular iron homeostasis, can be
critical in the osteogenic differentiation of beagle dog BMSCs induced by rhBMP-2.