Abstract: Objective To investigate the mechanism of β-amyloid protein (Aβ) in regulating the expression of the receptor for
advanced glycation end products (RAGE). Methods Aβ1-40 was injected into the bilateral hippocampus of rats, and 3 weeks
later, the levels of reactive oxygen species (ROS) production were detected by flow cytometry. The expressions of RAGE,
reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (gp9lphox and p47phox), nuclear factor-κB (NF-κB), and
inhibitor of κB (IκB) were measured by Western blotting. Results Injection of Aβ1-40 caused a significant increase in the
expressions of RAGE, gp9lphox, p47phox, phospho-p47phox, phospho-IκBα, NF-κB and phospho-NF-κB in rat hippocampus but
decreased the level of IκBα. Aβ1-40 injection also resulted in a significantly increased content of ROS in the hippocampus of the
rats. Conclusion Aβ up-regulates the expression of RAGE in rat hippocampus via NADPH/ ROS/NF-κB signaling pathway.