Abstract: ObjectiveTo investigate the incidence of human papillomavirus type16(HPV16) infection in patients with cervical
diseases and analyze E2gene mutations and its correlation with cervical lesions.MethodsSpecific primers were designed
according to the full-length E2gene sequence of HPV16from the GenBank. PCR was used to analyze the incidence of HPV16
infection in the exfoliated cell samples from 379patients with different cervical diseases positive for high-risk HPV. The
polymorphisms of E2gene of HPV16were analyzed by high-resolution melting.ResultsOf the379exfoliated cell samples
from the cervix, 78were positive for HPV16infection. The positivity rates of HPV16infection was 44.8% in patients with
cervical cancer,31.5% in patients with CIN II-III,24.1% in patients with CIN I and9.6% in patients with cervical inflammation.
The frequencies of E2gene68C and133G variations were significantly higher in patients with CIN II-III and those with
cervical cancer than in those with CIN I and those with cervical inflammation (P<0.05). ConclusionHPV16is an important
cause of cervical cancer, and HPV16infection rate increases with the pathological progression of the cervical lesions. The
naturally occurring sequence variations of HPV16E2gene may cause alterations of the carcinogenic potential of the virus.