Abstract: ObjectiveTo investigate the expression of casein kinase2βinesophageal carcinomatissuesandanalyzeitsclinical
significance.MethodsThe expression of CK2βinatissuechipcontaining60normal esophageal mucosa and60colorectal
cancer specimens were detected immunohistochemically. Ten human esophageal carcinoma and adjacent normal tissues were
examined for the expression of CK2βproteinandmRNAusingWesternblottingandreal-timequantitativePCR, respectively.
ResultsA strong expression of CK2βwasfoundin85.71% of the esophageal cancer tissues;1.79% of the cancer tissues were
negative for CK2βexpression, and1.79% and10.71% of the cancer tissues were weakly and moderately positive, respectively.
In the normal mucosal tissues, 96.67% of the tissues were negative for CK2βand3.33% showed weak CK2βexpression,
showing a significant difference in the distribution of CK2βbetweennormal andesophageal carcinomatissues(P<0.001). The
expression level of CK2βinesophageal cancerswasassociatedwiththepathological stage(TNM)(P=0.010). Western blotting
and real-time quantitative PCR also confirmed an increased CK2βexpressionintheesophagealcancertissues. ConclusionThe
high expression of protein kinase CK2βiscloselyrelatedtothecarcinogenesisandmalignancyofesophagealcancer.