Abstract: Objective To observe the sodium current of acutely isolated alveolar type Ⅱ cells and its regulation. Methods After isolation the AT Ⅱ cells, the whole cell sodium current were recorded in 5 hours with patch-clamp in whole-cell mode, and the effect of amiloride and terbutaline on the current was investigated. Results The amiloride-sensitive current of AT Ⅱ cells were recorded, which could be obviously stimulated by terbutaline. Conclusion Acutely isolated AT Ⅱ cells can be valuable for studying electrophysiological characteristics of sodium channels even under pathological conditions.