Abstract: Objective To investigate the expressions of lysophosphatidic acid (LPA) receptors at both mRNA and protein levels and their biological significance in different human ovarian cancer cell lines. Methods Reverse transcription-polymerase chain reaction (RT-PCR), streptovidin peroxidase-conjugated (SP) immunohistochemical assay and Western blotting were employed to measure the expression levels of LPA₁, LPA₂ and LPA₃ mRNA and LPA₂ and LPA₃ protein in cultured human ovarian cancer cell lines 3AO·SKOV3, and OVCAR3. Results All the ovarian cancer cell lines expressed LPA₁, LPA₂ and LPA₃ mRNAs and LPA₂ and LPA₃ proteins. Conclusion LPA₁, LPA₂ and LPA₃ expressions occur extensively in human epithelial ovarian cancer cell lines, and LPA₂ and LPA₃ may be involved in the development and progression of human ovarian cancer.