Oxidative stress-induced differentiation of L6 myoblasts

Abstract

Abstract: Objective To explore the relationship between the differentiation of L6 myoblasts and oxidative stress. Methods MTT assay was used to determine the viability of L6 myoblasts, from which the total RNA was extracted for amplification of the myogenin gene fragment by RT-PCR. H₂O₂-induced morphological changes of the cells were observed. Results The myoblasts treated with low concentration of reactive oxygen (50 μmol/L H₂O₂) for 1 h exhibited accelerated cell growth (P<0.05), and treatment with 50 and 150 μmol/L H₂O₂ induced the gene expression of myogenin, a molecular marker for differentiation of myoblasts. Morphological study revealed myotube formation and accelerated differentiation of the myoblasts induced by H₂O₂. Conclusion The reactive oxygen may serve as the intracellular signal molecules to induce the growth and differentiation of the myoblasts.

CLC Number:

R-33

QIU Xiao-zhong¹, YU Lei¹, LIAO Hua¹, ZHANG Li-sheng², QIN Jian-qiang¹, YANG Jun¹, OUYANG Jun¹. Oxidative stress-induced differentiation of L6 myoblasts[J]. Journal of Southern Medical University, 2005, 25(11): 1384-1386.

References

[1] Weissman I. Translating stem and progenitor cell biology to the clinic:barriers and opportunities[J]. Science, 2000, 287: 1441-6.
[2] Peus D, Beyerle A, Vasa M, et al. Antipsoriatic drug anthralin induces EGF receptor phosphorylation in keratinocytes: requirement for H₂O₂ generation[J]. Exp Dermatol, 2004, 13(2): 78-85.
[3] Heslop L, Beauchamp JR, Tajbakhsh S, et al. Transplanted primary neonatal myoblasts can give rise to functional satellite cells as identified using the MyfSnlacZl+ mouse[J]. Gene Ther, 2001, 8(10):778-83.
[4] Morita N, Iizuka K, Okita K, et al. Exposure to pressure stimulus enhances succinate dehydrogenase activity in L6 myoblasts[J]. Am J Physiol Endocrinol Metab, 2004, 287(6): E1064-9.
[5] Bardouille C, Vullhorst D, Jockusch H. Expression of chloride channel 1 mRNA in cultured myogenic cells: a marker ofmyotube maturation[J]. FEBS Lett, 1996, 396: 177-80.
[6] Buckingham ME. Actin and myosin multigene families: their expression duringthe formation of skeletal muscle[J]. Essays Biochem,1985, 20: 77-109.
[7] Kim JA, Jonsson CB, Calderone T, et al. Transcription of MyoD and myogenin in the non-contractile electrogenic cells of the weakly electric fish, Sternopygus macrurus[J]. Dev Genes Evol, 2004, 214(8): 380-92.
[8] Michelagnoli MP, Burchill SA, Cullinane C, et al. Myogenin——a more specific target for RT-PCR detection of rhabdomyosarcoma than MyoD1[J]. Med Pediatr Oncol, 2003, 40(1): 1-8.
[9] Guan Y,Taylor J, Jane M. p130/p107 expression distinguishes adipogenic potential in primary myoblasts based on age[J]. Biochem Biophys Res Commun,2002,6, 296(5): 1340-5.
[10] Chakravarthy MV, Booth FW, Spangenburg EE.The molecular responses of skeletal muscle satellite cells to continuous expression of IGF-1: implications for the rescue of induced muscular atrophy in aged rats[J]. Iht J Sport Nutr Exerc Metab, 2001, 11 (Suppl): 44-8.
[11] Charge SB, Brack AS, Hughes SM, et al. Aging-related satellite cell differentiation defect occurs prematurely after Ski-induced muscle hypertrophy[J]. Am J Physiol Cell Physiol, 2002, 283(4): C1228-41.
[12] Orzechowski A, Grizard J, Jank M, et al. Dexamethasone-mediated regulation of death and differentiation of muscle cells. Is hydrogen peroxide involved in the process[J] ? Reprod Nutr, 2002, 42 (3):197-216.

[1]	YUAN Qiang, LIU Da-lie, YUAN Ji-long, SHAN Lei Department of Plastic and Reconstructive Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. Establishment of rabbit orbital hypertelorism model and evaluation of effects of surgical correction [J]. Journal of Southern Medical University, 2006, 26(10): 1476-.
[2]	CUI Yan-zhi1, HAN Ya-guang1, LUO Rong-cheng1, YANG Hai-qing2, JIANG Da3 1Department of Oncology, Nanfang Hospital, 3Department of Statistics, Southern Medical University, Guangzhou 510515, China; 2Department of Oncology, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China. Establishment of an rat model of tumor-associated depression [J]. Journal of Southern Medical University, 2006, 26(10): 1513-.
[3]	. [J]. Journal of Southern Medical University, 2006, 26(08): 1209-1210.
[4]	LI Hao, PENG Han-wei, ZENG Zong-yuan, GUO Zhu-ming State Key Laboratory of Oncology in Southern China, Department of Head and Neck, Oncology Center, Sun Yat-sen University, Guangzhou 510060, China. Research of modified rat laryngeal transplantation model [J]. Journal of Southern Medical University, 2006, 26(07): 994-.
[5]	. [J]. Journal of Southern Medical University, 2006, 26(07): 1069-1070.
[6]	CHEN Xiao-lin,HE Xiao-hong,ZHANG Yan,QIAN Yue-tao,LIANG Lu-guang,FANG Dian-qiu,ZHAN Cheng-yang,ZHENG Zhen-sheng,MA Hong Department of Cardiology,First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510089,China. Establishment of a pig model of chronic ally enhanced external counterpulsation [J]. Journal of Southern Medical University, 2006, 26(05): 613-614.
[7]	DONG Gao-hong1,ZHANG Zhao-da2,HU Wei-ming2,TANG Yong2,LI Jian-shui2 1Center of Organ Transplantation,First Affiliated Hospital of Jinan University,Guangzhou 510630,China;2West China Hospital of Sichuan University,Chengdu 610041,China. Establishment of whole pancreaticoduodenal allotransplantation model with portal venous drainage and enteric drainage in pigs [J]. Journal of Southern Medical University, 2006, 26(05): 626-628.
[8]	LI qi1,HUANG De-liang2 1Institute of Otolaryngology,2Department of Otolaryngology-Head and Neck Surgery,General Hospital of PLA,Beijing 100853,China. Expression of aquaporin-1 in a guinea pig model of labyrinth destruction [J]. Journal of Southern Medical University, 2006, 26(05): 664-666.
[9]	. [J]. Journal of Southern Medical University, 2006, 26(05): 707-708.
[10]	LIN Xiao-jing, ZOU Fei, LI Ya-jie, WANG Bin, HE Wen-jing, ZHAO Zhi-rong, ZHU Shun-fang Nanfang Hospital, Institute of Public Hygiene and Tropical Medicine, Southern Medical University, Guangzhou 510515, China. Establishment of a rat model of severe heatstroke complicated with endotoxemia [J]. Journal of Southern Medical University, 2006, 26(01): 86-89.
[11]	XIONG Bing¹, CHEN Hua-de¹, LAI Wen¹, XIONG Shi-qiu², ZHEN Shao-yi¹, GAO Hui¹, BIAN Hui-ning¹, LIU Zu-an¹. Establishing a parabiosis animal model of two-way paradigm [J]. Journal of Southern Medical University, 2005, 25(12): 1511-1513,1516.
[12]	FU Rui-bin¹, WU Ping-sheng², SONG Yun-feng¹, QIU Jian¹, DAI Tie-ying², LI Jian-hua², XIU Jian-cheng². Construction of eukaryotic expression vectors of two mutants of hypoxia-inducible factor-1 and their expressions in human microvascular endothelial cells [J]. Journal of Southern Medical University, 2005, 25(11): 1348-1351.
[13]	HU Jing¹, YU Shou-yi¹, KAN Biao², LIU Zhi-hua³. Construction and characterization of mutant Enteroaggregative E. coli O42 strain with high-pathogenicity island deletion [J]. Journal of Southern Medical University, 2005, 25(11): 1352-1356.
[14]	DENG Hong, ZOU Fei, LUO Hai-ji. Differentiation of neonatal rat striatal neural stem cells induced by all-trans retinoic acid [J]. Journal of Southern Medical University, 2005, 25(11): 1357-1360,1374.
[15]	WANG Ping¹, WU Xiao-hua¹, CHEN Wen-xue¹, SHAN Bao-en², GUO Qing³. Expression of lysophosphatidic acid receptor in human ovarian cancer cell lines 3AO,SKOV3,OVCAR3 and its significance [J]. Journal of Southern Medical University, 2005, 25(11): 1422-1424,1431.