Abstract: Objective To evaluate the accuracy of contrast-enhanced ultrasound (CEU) in renal blood flow quantification. Methods Regional renal perfusion was quantified with CEU in 10 dogs at the baseline level and after treatment with 3 doses of dopamine (3, 8 and 16 mg·kg^-1·min^-1), and renal arterial flow (RAF) was measured with ultrasonic flow probes deployed directly on the renal artery simultaneously. Normalized RAF was calculated as RAF divided by renal weight (ml·min^-1·g^-1). Results Compared with the baseline level, a progressive increase in RAF was induced by dopamine treatment at the doses of 3 and 8 mg·kg^-1·min^-1 (P<0.05), but at a higher dose of 16 mg·kg^-1·min^-1, the increment in RAF was reduced in comparison with that with the two lower doses (P<0.05). The same changes in cortical nutrient blood flow derived from CEU were observed. Significant positive correlation was found between normalized RAF and CEU-derived cortical nutrient blood flow (y=39.8x +44.3, r=0.88, P<0.001). Conclusion CEU can be used to accurately quantify renal blood flow in dogs.