Cloning of human obesity gene and its expression in E. coli

Abstract

Abstract: Objective To clone the obesity gene of Chinese and express human leptin in E. coli. Method The obesity gene was amplified from the total RNA isolated from cultured human adipocytes of Chinese by reverse transcriptional PCR, inserted into TA-vector and cloned into the expression plasmid pBV220 after sequence identification. Results DNA sequencing confirmed that the isolated obesity gene was identical to the previously reported sequence. The recombinant plasmid pBV220-OB was constructed and leptin successfully expressed in E. coli. Conclusion Successful cloning and expression of human obesity gene in E. coli may facilitate further research of the mechanism of fat metabolism and adipocyte differentiation.

CLC Number:

Q785

ZHU Ji^1,2, MA Wen-li¹, MAO Xiang-ming¹, LI Ling¹, SONG Yan-bin¹, ZHENG Wen-ling³. Cloning of human obesity gene and its expression in E. coli[J]. Journal of Southern Medical University, 2005, 25(04): 395-398.

References

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