Effect of adenosine A1-receptors on the respiratory rhythmical discharge activity in neonatal rat brainstem slice in vitro

Abstract

Abstract: Objective To explore the role of adenosine A₁-receptors in the generation and regulation of central respiratory rhythm. Methods Neonatal rat brainstem slices including the medial region of the nucleus retrofacialis (mNRF) with the hypoglossal nerve rootlets retained were prepared in which the respiratory activity was recorded from the hypoglossal nerve rootlets by suction electrode. The possible role of adenosine A₁-receptors on rhythmical respiration was investigated by administration of specific antagonist of adenosine A₁-receptor R-phenylisopropyl-adenosine (R-PIA) and its antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) in modified Kreb’s solution (MKS) for perfusion of the brainstem slices.Results DPCPX induced a significant decrease in the expiratory time and respiratory cycles. However, R-PIA significantly prolonged the respiratory cycle and decreased the inspiratory time and the integral amplitude. The effect of R-PIA on the rhythmical discharges could be partially reversed by additional application of DPCPX.Conclusion Adenosine A₁-receptors are involved in the modulation of rhythmical respiratory in neonate rat brainstem.

CLC Number:

Q42
R338.2

WANG Jian-Li, WU Zhong-Hai. Effect of adenosine A₁-receptors on the respiratory rhythmical discharge activity in neonatal rat brainstem slice in vitro[J]. Journal of Southern Medical University, 2005, 25(03): 277-280.

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Effect of adenosine A₁-receptors on the respiratory rhythmical discharge activity in neonatal rat brainstem slice in vitro

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