Transient cytosolic free calcium changes in cultured neonatal rat cardiac myocytes in response to advanced glycation end-product treatment

Abstract

Abstract: Objective To investigate the effects of advanced glycation end-products (AGEs) on transient cytosolic free calcium in neonatal rat cardiac myocytes (CMs) cultured in vitro.Methods CMs cultured for 3 to 5 days in vitro were incubated with Ca²⁺-sensitive fluorescent indicator Fluo-3AM with light screening at 37℃ with 5% CO₂ for 60 min. Changes of the fluorescence signal of free calcium caused by AGEs were measured under laser scanning confocal microscope (LSCM).Results Compared with the control cells, AGEs caused an increase in the concentration of cytosolic free calcium in a dose-dependent manner.Conclusion AGEs may impair neonatal rat CMs by altering cytosolic free calcium concentration.

CLC Number:

SHAO Ya-hui¹, XU Ding-li¹, ZENG Ping¹, LAI Wen-yan², REN Hao¹, JIANG Rong-yan¹. Transient cytosolic free calcium changes in cultured neonatal rat cardiac myocytes in response to advanced glycation end-product treatment[J]. Journal of Southern Medical University, 2005, 25(03): 274-276,280.

References

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