Effects of macrophages on the biological behaviors and VEGF receptor mRNA, Hoxb2 mRNA, and integrin ανβ3 expressions of vascular endothelial cells

Abstract

Abstract: Objective To explore the mechanism by which macrophages regulate angiogenesis by co-culturing human umbilical vein endothelial cells(ECV-304) with human macrophage cells(U937) stimulated by concanavalin A(ConA). Methods Monolayer ECV-304 cells growing to 60% confluence were co-cultured with 1×105/ml U937 cells in the presence or absence of ConA(ConA+U937+ECV-304 and U937+ECV-304 groups, respectively), with non-treated and ConA-treated ECV-304 cells serving as the control groups(ECV-304 and ConA+ECV-304 groups, respectively). Forty-eight h later, U937 cells were removed from the cell co-culture for examining changes in DNA synthesis of ECV-304 cells with 3H-TdR incorporation assay and for cell cycle analysis with flow cytometry. RT-PCR was employed to assess the influence of macrophages stimulated by ConA on the expression of the target genes. With immunofluorescent method, the changes in the expression of integrin receptor ανβ3 of ECV-304 were determined. Results A significant increase in S-phase ECV-304 cells with enhanced DNA synthesis was observed after co-culture of the cells with ConA-stimulated U937 cells(P<0.01), which also resulted in significant up-regulation of the expressions of KDR mRNA(0.879±0.003), Hoxb2 mRNA(0.947±0.003) and integrin receptor ανβ3(10.26±1.73). Conclusion Macrophages can accelerate the proliferation, migration and adhesion of the vascular endothelial cells to the basilar membrane matrix by affecting their cell cycle, DNA synthesis, expression of KDR mRNA, Hoxb2 mRNA and integrin ανβ3, so as to modulate the angiogenetic process of the latter cells.

CLC Number:

R329.28

LIU Liang¹, LIU Xu-sheng², ZHANG Xiao-qi³, MING Jia², XU Hui⁴, CHENG Tian-min⁴. Effects of macrophages on the biological behaviors and VEGF receptor mRNA, Hoxb2 mRNA, and integrin ανβ3 expressions of vascular endothelial cells[J]. Journal of Southern Medical University, 2005, 25(02): 148-151.

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