Effects of a novel recombinant polypeptide from the sea anemone Anthopleura sp. on left ventricular function in dogs with acute cardiac insufficiency

Journal of Southern Medical University ›› 2005, Vol. 25 ›› Issue (01): 37-39,43.

Effects of a novel recombinant polypeptide from the sea anemone Anthopleura sp. on left ventricular function in dogs with acute cardiac insufficiency

OUYANG Ping¹, XIE Jin-guo¹, LIU Jian¹, XIAO Wen-xing¹, LIU Yan-bo¹, WANG Lei², LIANG Dong², WANG Yong-hua², XU Ding-li¹, LIU Yi-li¹, XU An-long

1. 南方医科大学南方医院心血管内科, 广东广州510515;
2. 中山大学生命科学学院国家高技术计划海洋生物功能基因组开放实验室, 广东广州510275

Online:2005-01-20 Published:2005-01-20

Abstract

Abstract: Objective To observe the effects of recombinant hk2a, a novel neurotoxin from the sea anemone Anthopleura sp., on left ventricular function of dogs with acute cardiac insufficiency. Methods Canine models of acute cardiac insufficiency were established by rapid ventricular pacing, in which the left ventricular ejection fraction (LVEF) was measured by Acuson ultrasound systems (Sequoia 512) at 0, 5, 15, 30 and 60 min, respectively, after intravenous injection of 30 μg/kg recombinant hk2a. The response of the canine models to hk2a treatments was observed in comparison with that of the dogs treated with Cedilanid (as positive control) and saline (as negative control). Results Intravenous injection of recombinant hk2a caused an immediate and significant increase in LVEF in the canine models of acute cardiac insufficiency (P<0.05), and the effect maintained for more than 30 min without significant effect on heart rate. Recombinant hk2a possessed such merits as quicker onset and greater potency in comparison with Cedilanid. Conclusion Recombinant hk2a may significantly increase LVEF of the dogs with acute cardiac insufficiency.

CLC Number:

R331.3⁺1

OUYANG Ping¹, XIE Jin-guo¹, LIU Jian¹, XIAO Wen-xing¹, LIU Yan-bo¹, WANG Lei², LIANG Dong², WANG Yong-hua², XU Ding-li¹, LIU Yi-li¹, XU An-long. Effects of a novel recombinant polypeptide from the sea anemone Anthopleura sp. on left ventricular function in dogs with acute cardiac insufficiency[J]. Journal of Southern Medical University, 2005, 25(01): 37-39,43.

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