C-reactive protein inhibits the expression of hypoxia-inducible factor-1α in human umbilical vein endothelial cells in hypoxic condition

Abstract

Abstract: Objective To investigate the effect of C-reactive protein (CRP) on expression of hypoxia-inducible factor-1 (HIF-1). Methods Using COCl₂ (100 mol/L) to simulate hypoxic condition for culturing human umbilical vein endothelial cells (HUVECs), we examined the effects of CRP on expression of HIF-1α. The proteins were extracted from the cells after a 24-hour exposure of the cells to CRP of varied concentrations in the presence of COCl₂, and Western blotting was performed for quantification of HIF-1α expression, the results were analyzed statistically with SPSS software. Results CRP at the concentration of 5 μg/ml decreased the expression ofHIF-1α (P<0.001), producing the maximum inhibitory effect at the concentration of 100μg/ml, an effect exhibiting dose-dependence. Conclusion CRP inhibits the expression of HIF-1α in HUVECs subjected to hypoxic condition, which serves as an important evidence for the inhibitory effect of CRP on angiogenesis in hypoxic condition.

CLC Number:

R54
R-33

SONG Yun-feng, WU Ping-sheng, LAI Wen-yan. C-reactive protein inhibits the expression of hypoxia-inducible factor-1α in human umbilical vein endothelial cells in hypoxic condition[J]. Journal of Southern Medical University, 2004, 24(07): 746-748.

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