Differences in gene expression profile between high and low metastatic human colorectal cancer cell lines

Abstract

Abstract: Objective To analyze the gene expression profiles in colorectal cancer cell lines including high metastatic LoVo and SW620 cell lines and low metastatic SW480 and LS174T cells lines using cDNA microarray for tumor metastasis-associated gene and to screen specific colorectal cancer metastasis-associated genes. Methods The cDNAs reversely transcribed from the mRNA derived respectively from the 4 cell lines and normal colorectal tissues were labeled with cy3 and cy5 prior to hybridization with the microarray containing 399 genes. The acquired image was analyzed by software. Results There were significant differences in gene expression profiles between high- and low- metastatic cell lines, and 44 up-regulated genes and 60 down-regulated genes were found to have statistical significance. Conclusions The complex alterations in gene expression underlie the development of metastasis of colorectal cancer. The genes that are expressed differentially in high- and low- metastatic cell lines are probably related to the high metastatic potentials.

CLC Number:

Q785

WENG De-sheng¹, DING Yan-qing¹, SUN Qing². Differences in gene expression profile between high and low metastatic human colorectal cancer cell lines[J]. Journal of Southern Medical University, 2004, 24(03): 256-259.

References

[1] Nelson H, Petrelli N, Carlin A. Guidelines 2000 for colon and rectal cancer surgery[J]. J Natl Cancer Inst, 2001, 93(8): 583-96.
[2] Drewinko B, Romsdahl MM, Yang LY, et al. Establishment ofa hu man carcinoembryonic antigen-producing colon adenocarcinoma cell line[J]. Cancer Res, 1976, 36(2 Pt 1): 467-75.
[3] McNutt NS, Mak LL, Kim YS.Comparison of cell peripheries in the human colonic adenocarcinoma cell lines SW480 and SW620 grown in floating chamber culture, cover slip culture, athymic (nude) mice, and BALB/c mice[J]. Lab Invest, 1981, 44(4): 309-23.
[4] Tom BH, Rutzky LP, Oyasu R, et al. Human colon adenocarcinoma cells. Ⅱ. Tumorigenic and organoid expression in vivo and in vitro[J]. J Natl Cancer Inst, 1977, 58(5): 1507-12.
[5] Mareel M. When and why does cancer metastasize? An overview of current viewpoints of the molecular mechanism of invasiveness [J]. Verh K Acad Geneeskd Belg, 1994, 2: 105-25.
[6] Baselga J. The EGFR as a target for anticancer therapy--focus on ce tuximab[J]. Eur J Cancer, 2001, 37(Suppl 4): 516-22.
[7] Natsume T, Koh Y, Kobayashi M, et al. Enhanced antitumor activi ties of TZT-1027 against TNF-beta or IL-6 secreting Lewis lung car cinoma in vivo [J]. Chemother pharmacol, 2002, 49( 1 ): 35-47.
[8] Cavallaro U, Schaffhauser B, Christofori G. Cadherins and the tu mour progression: is it all in a switch[J]? Cancer Lett, 2002, 176 (2): 123-8.
[9] Alexiou D, Karayiannakis AJ, Syrigos KN, et al. Serum levels of E-selectin, ICAM-1 and VCAM-1 in colorectal cancer patients: cor relations with clinicopathological features, patient survival and tu rnour surgery[J]. Eur J Cancer, 2001, 37(18): 2392-7.
[10] 李祖国,丁彦青,邓永键,等.CD44V6对实验性大肠癌肝转移的影响[J].中国肿瘤临床(Chin JClinOncol),1999,26(2):139-42.
[11] Kostoulas G, Lang A, Nagase H, et al. Stimulation of angiogenesisthrough cathepsin B inactivation of the tissue inhibitors of matrix metalloproteinases[J]. FEBS Lett, 1999, 455(3): 286-90.
[12] Sargiannidou I, Zhou J, Tuszynski GP. The role of thrombospondin 1 in tumor progression [J]. Exp Biol Med (Maywood), 2001, 226(8): 726-33.
[13] Cui Q, Takiguchi S, Matsusue K, et al. Assignment of the hman metastasis-associated gene 1 (MTA1) to human chromosome band14q32.3 by fluorescence in situ hybridization [J]. Cytogenet Cell Genet, 2001, 93(1-2): 139-40.
[14] del Pozo MA, Vicente Manzanares M, Tejedor R. Rho GTPases control migration and polarization of adhesion molecules and cytoskele tal ERM components in T lymphocytes[J]. Eur J Immunol, 1999,29(11): 3609-20.
[15] Tomita M, Ayabe T, Matsuzaki Y. Expression ofnm23-H1 gene product in mediastinal lymph nodes from lung cancer patients [J]. Eur J Cardiothorac Surg, 2001, 19(6): 904-7.