南方医科大学学报

南方医科大学学报 ›› 2014, Vol. 34 ›› Issue (10): 1503-.

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壳聚糖纳米粒介导的PIK3CA基因干扰对胃癌细胞侵袭转移能力的影响

周新科，何璐，梁敏，刘季芳   

  • 出版日期:2014-10-20 发布日期:2014-10-20

Chitosan/PIK3CA siRNA nanoparticle-mediated PIK3CA gene interference decreases the
invasive capacity of gastric cancer cells in vitro

  • Online:2014-10-20 Published:2014-10-20

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摘要： 目的观察PIK3CA siRNA-壳聚糖纳米粒子处理胃癌细胞BGC823后，胃癌细胞BGC823侵袭能力的变化，研究壳聚糖纳
米粒子介导PIK3CA干扰在抑制胃癌侵袭转移中的应用价值。方法使用壳聚糖包被PIK3CA siRNA制备纳米粒子，纳米粒子
粒径在350 nm。用其处理胃癌细胞，通过western blot及PCR检测PIK3CA沉默情况，并通过Transwell实验观察细胞侵袭性的
变化。结果PIK3CA siRNA-壳聚糖纳米粒子可显著下调胃癌细胞BGC823中PIK3CA的表达（P<0.01），并且可明显抑制胃癌
细胞的侵袭性（P<0.001）。结论通过壳聚糖纳米粒子介导的PIK3CA基因干扰可以显著降低胃癌细胞的侵袭能力。

Abstract: Objective To investigate the effect of PIK3CA/siRNA chitosan nanoparticle on the invasiveness of gastric carcinoma
and the potential value of PIK3CA/siRNA chitosan nanoparticle in suppressing the metastasis of gastric carcinoma. Methods
Gastric cancer cells were treated with PIK3CA/siRNA nanoparticle (with a diameter of 350 nm), and the efficiency of PIK3CA
gene interference was evaluated using Western blotting and real-time PCR. The changes of the invasive capacity of the treated
cells was assessed with Transwell assay. Results PIK3CA/siRNA chitosan nanoparticle efficiently lowered the expression level
of PIK3CA and significantly decreased the invasion of BGC823 cells. Conclusion PIK3CA gene interference mediated by
PIK3CA/siRNA chitosan nanoparticle can decrease the invasive capacity of gastric cancer cells in vitro.