南方医科大学学报

南方医科大学学报 ›› 2014, Vol. 34 ›› Issue (10): 1420-.

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白纹伊蚊嗅觉受体OR7的基因克隆、鉴定和功能分析

周宁，王晓明，邓裕华，刘宏美，刘通，陈晓光   

  • 出版日期:2014-10-20 发布日期:2014-10-20

Cloning, identification and preliminary functional analysis of odorant receptor 7 gene of
Aedes albopictus

  • Online:2014-10-20 Published:2014-10-20

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摘要： 目的克隆鉴定白纹伊蚊嗅觉受体基因OR7，并对其表达谱和功能进行分析。方法提取成蚊总RNA，采用RT - PCR技
术扩增白纹伊蚊嗅觉受体基因OR7的全长编码基因，并检测OR7在不同虫期和不同组织器官中的表达谱；将OR7基因克隆于
真核表达载体pME18s中，转染HEK293细胞，利用钙成像技术初步分析其对气味分子刺激的钙调功能。结果成功克隆出白纹
伊蚊嗅觉受体基因OR7的全长编码基因，经测序得知其编码区序列长度为1395 bp；表达谱分析显示，其在蚊幼虫、蛹、成蚊中
都有表达，且在雌蚊嗅器中表达显著。钙成像结果显示OR7对气味分子刺激后的钙离子通道有一定的调节功能。结论克隆出
白纹伊蚊的嗅觉受体基因OR7 ，初步分析显示其在雌蚊中显著表达，并具有对气味分子的识别功能。

Abstract: Objective To clone and identify olfactory receptor odorant receptor 7 (OR7) gene of Aedes albopictus and analyze its
expression profile and calcium regulation function. Method RT-PCR was used to amplify the olfactory receptor OR7 gene of
Ae. albopictus and OR7 expression was detected in different tissues and organs. The coding sequence of OR7 gene was cloned
in eukaryotic expression vector pME18s, which was then transfected into HEK293 cells. The calcium callback function in
response to odor molecule stimulation was analyzed by calcium imaging technique. Results The OR7 gene of Ae. albopictus
was cloned and sequence analysis showed that its coding region was 1395 bp. RT-PCR detected OR7 expression in the larvae,
pupae and adult mosquitoes, especially in female mosquitos. Preliminary analysis of calcium callback function demonstrated
the specific regulation of calcium absorption by OR7 in response to odor molecule stimulation. Conclusion The OR7 gene of
Ae. albopictus has been cloned successfully. OR7 is highly expressed in female mosquitos and is capable of specific recognition
of the odor molecules.