Abstract: Objective To study the influence of hepatitis B virus (HBV) replication and expressions of different viral genes on
CDC37 level in hepatocytes. Methods We amplified and cloned 6 HBV genes (P, preS1, preS2, S, C and X) into pCMV
expression vectors, which were transfected in Huh7 and HepG2 hepatoma cell lines, and CDC37 expression level in the cells
was detected using Western blotting. W also cloned the promoter sequence of CDC37 into pGL3 vector, and co-transfected
pGL3 with pCMV recombinant plasmids into Huh7 and HepG2 cells and the fluorescent signals were detected. To study the
influence of HBV replication on CDC37 expression, we constructed 1.28-copy overlength genome of HBV genotypes B, C, D
and CD recombinant. The overlength HBV genome was transformed into Adeasier-1 cells for recombination and into 293 cells
for packaging. Huh7 and HepG2 cell lines infected with the packaged HBV recombinant adenoviruses were examined for
CDC37 expression with Western blotting. Results Western blotting showed that the expression of different HBV genes did not
obviously affect the protein level of CDC37 in the hepatocytes. The protein expression of HBV genes had no effect on the
activity of CDC37 promoter. Huh7 and HepG2 cells infected with 1.28-copy HBV replicon showed no significant changes in
the expression level of CDC37. Conclusion HBV replication and its gene expression have no effect on the level of CDC37 in
hepatocytes in vitro.