南方医科大学学报

南方医科大学学报 ›› 2014, Vol. 34 ›› Issue (01): 31-.

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miR-124a通过抑制 iASPP基因表达促进神经突起生长

林利芳，谷 溪，刘书虎，王雪敏   

  • 出版日期:2014-01-20 发布日期:2014-01-20

miR-124a promotes neurite outgrowth by inhibiting iASPP expression

  • Online:2014-01-20 Published:2014-01-20

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摘要： 目的探索miR-124a的靶基因iASPP对神经发育的影响。方法利用在线预测软件TargetScan对miR-124a的靶基因进行
预测，结合文献资料提到的与神经早期发育有关的基因进行筛选，我们选择iASPP作为miR-124a的候选靶基因。通过荧光素
酶报告基因实验对其进行验证，然后在M17细胞中转染miR-124a过表达质粒，用Western blotting实验检测iASPP蛋白表达水
平的变化。利用视黄酸诱导M17细胞作为神经元分化模型，通过过表达或者基因干扰的方法，初步探讨iASPP基因对神经发育
的影响。结果miR-124a促进神经突起发育，并且能够与iASPP基因的3’非翻译区（3’UTR）相互作用来抑制其蛋白质的表达；
iASPP基因的过表达抑制神经突起的发育并且抑制miR-124a促进神经突起生长的作用。结论miR-124a能够通过抑制iASPP
基因的表达来促进神经突起生长。

Abstract: Objective To investigate the role of iASPP as the target gene of miR-124a in neural development. Methods Using the
online bioinformatical tool (TargetScan) and by reviewing the relevant studies, we selected iASPP as the candidate target gene
of miR-124a involved in early-stage neuronal differentiation. Luciferase reporter assay was used to verify the candidate gene.
We transfected M17 cells with a miR-124a overexpression plasmid and detected the changes in the protein expression of iASPP
using Western blotting. With retinoic acid-induced M17 cells as the neuronal differentiation model, the role of iASPP in
early-stage neuronal differentiation was investigated by gene overexpression and gene interference techniques. Results
miR-124a inhibited the expression of iASPP in M17 cells by interacting with the 3’UTR of iASPP gene. miR-124a promoted
neurite outgrowth of the cells, which was blocked by iASPP overexpression. Conclusion miR-124a promotes neurite
outgrowth of M17 cells by inhibiting iASPP expression.