南方医科大学学报

南方医科大学学报 ›› 2012, Vol. 32 ›› Issue (10): 1503-.

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宫颈病变患者人乳头瘤病毒16亚型E2基因多态性检测

邱峰，袁丽娜，黄宪章   

  • 出版日期:2012-10-20 发布日期:2012-10-20

Detectionof E2gene polymorphism of human papillomavirus type16in patients with cervical lesions and cervical cancer

  • Online:2012-10-20 Published:2012-10-20

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摘要： 目的分析宫颈病变中人乳头瘤病毒（HPV）16亚型E2基因的多态与宫颈病变的关系，了解E2基因变异情况及其与宫颈
病变的相关性。方法应用PCR和高分辨率熔解曲线方法针对379例HPV高危亚型阳性宫颈脱落细胞样本进行HPV16感染
情况及HPV16亚型E2基因68位点和133位点多态分布情况进行检测。结果379例HPV高危亚型阳性宫颈标本共检出78例
HPV16亚型阳性，其在宫颈癌、CINⅡ~Ⅲ、CINⅠ、炎症患者中的检出率分别为44.8%、31.5%、24.1%和9.6%；HPV16E2基因
68C和133G的频率在中重度宫颈内瘤样病变和宫颈癌中明显高于轻度上皮内瘤样病变和炎症患者（P<0.05）。结论HPV16是
导致宫颈恶性病变的重要致病因素；随着病理类型的进展，HPV16感染率也逐渐增加；同时基因位点改变说明HPV16E2基因
单核苷酸变异可能使致癌能力发生改变。

Abstract: ObjectiveTo investigate the incidence of human papillomavirus type16(HPV16) infection in patients with cervical
diseases and analyze E2gene mutations and its correlation with cervical lesions.MethodsSpecific primers were designed
according to the full-length E2gene sequence of HPV16from the GenBank. PCR was used to analyze the incidence of HPV16
infection in the exfoliated cell samples from 379patients with different cervical diseases positive for high-risk HPV. The
polymorphisms of E2gene of HPV16were analyzed by high-resolution melting.ResultsOf the379exfoliated cell samples
from the cervix, 78were positive for HPV16infection. The positivity rates of HPV16infection was 44.8% in patients with
cervical cancer,31.5% in patients with CIN II-III,24.1% in patients with CIN I and9.6% in patients with cervical inflammation.
The frequencies of E2gene68C and133G variations were significantly higher in patients with CIN II-III and those with
cervical cancer than in those with CIN I and those with cervical inflammation (P<0.05). ConclusionHPV16is an important
cause of cervical cancer, and HPV16infection rate increases with the pathological progression of the cervical lesions. The
naturally occurring sequence variations of HPV16E2gene may cause alterations of the carcinogenic potential of the virus.