南方医科大学学报

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (07): 820-822.

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水红花子水提物的抗氧化活性

雷晓燕1, 许爱霞2, 高湘2, 葛斌2   

  1. 1. 甘肃省人民医院小儿科, 甘肃, 兰州, 730000;
    2. 甘肃省人民医院药剂科, 甘肃, 兰州, 730000
  • 出版日期:2005-07-20 发布日期:2005-07-20
  • 基金资助:
    收稿日期:2005-1-12。
    基金项目:甘肃省自然科学基金(3ZS041-A25-073)
    作者简介:雷晓燕(1958- ),女,1981年毕业于兰州医学院,学士,主任医师,电话:0931-3912733
    通讯作者:葛斌,副主任药师,电话:0931-8281809,E-mail: gebin 1965 @sina.com

Antioxidative activity of water-soluble extracts from Fructus Polygoni Orientalis

LEI Xiao-yan1, XU Ai-xia2, GAO Xiang2, GE Bin2   

  1. 1. 甘肃省人民医院小儿科, 甘肃, 兰州, 730000;
    2. 甘肃省人民医院药剂科, 甘肃, 兰州, 730000
  • Online:2005-07-20 Published:2005-07-20

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摘要： 目的 研究水红花子水提物的抗氧化活性及其机制。方法 用D-半乳糖建立衰老小鼠模型,分别灌胃服用生药量0.8、1.6和3.2g/kg·b.w.的水红花子水提物(n=10),每日1次,共7周。分别用TBA比色法、亚硝酸盐法、DTNB法及Sohal法测定心、肝、肾中丙二醛(MDA)、超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)及脑中脂褐质(LF)含量。结果 衰老小鼠心、肝、肾组织中MDA含量升高,SOD及GSH-PX活性降低,脑组织中LF升高。上述3种剂量的EFPO,能不同程度抑制脑组织中LF升高及心、肝、肾组织中MDA升高,其量效关系均呈负相关,相关系数分别为-0.963(相关性检验P<0.01)、-0.981(P<0.01)、-0.966(P<0.05)及-0.978(P<0.01);能不同程度抑制心、肝、肾组织中SOD活力下降,其量效关系均呈正相关,相关系数分别为0.984(P<0.05)、0.990(P<0.01)、0.985(P<0.01);能不同程度抑制心、肝、肾组织中GSH-PX活力下降,其量效关系均呈正相关,相关系数分别为0.984(P<0.01)、0.956(P<0.01)及0.957(P<0.01)。结论 水红花子水提物可通过清除·OH,提高SOD及GSH-PX活力而发挥抗氧化活性。

Abstract: Objective To study the antioxidative activity of the water-soluble extracts from Fructus Polygoni Orientalis. Methods Malondialdehyde and superoxide dismutase were measured by thiobarbituric acid assay,nitrite method,respectively.The activity of glutathione peroxidase was determined by 5,5’-dithionbis (2-nitrobenzoic acid) photometric method.Lipofuscin was determined by Sohal method in the brain of senile model mice induced by D-galactose.The extracts of 0.8,1.6 and 3.2 g·kg-1>·d-1 were administered to the mice separately through oral gavage daily for 7 weeks. Results Malondialdehyde content increased,activities of superoxide dismutase and glutathione peroxidase in the heart,liver and kidney decreased.Levels of lipofuscin in the brain of senile model mice induced by D-galactose increased.The three doses of the extracts could inhibit the levels of lipofuscin in the brain,malondialdehyde in the heart,liver and kidney.These results showed that there was a negative dose-effect relationship.The extracts also up-regulated the levels of superoxide dismutase and glutathione peroxidase in the heart,liver and kidney with a positive dose-effect relationship. Conclusion The water-soluble extracts exert its antioxidative activity by eliminating free hydroxyl radicals,increasing activities of superoxide dismutase and glutathione peroxidase.

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