南方医科大学学报

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (12): 1565-1567.

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HelenaSAS-3全自动琼脂糖电泳仪在血红蛋白分析中的应用及评价

莫建坤, 张智, 黎永新, 雷达, 马洁   

  1. 广东省第二人民医院检验科, 广东, 广州, 510310
  • 出版日期:2005-12-20 发布日期:2005-12-20
  • 基金资助:
    收稿日期:2005-10-20。
    基金项目:广东省自然科学基金(04005052)
    作者简介:莫建坤(1962- ),男,2001年毕业于第三军医大学,本科,副主任技师,电话:020-89168132,E-mail:mojiankun@21cn.com.

Application of Helena SAS-3 full automatic hemoglobin agarose electrophoresis

MO Jian-kun, ZHANG Zhi, LI Yong-xin, LEI Da, MA Jie   

  1. 广东省第二人民医院检验科, 广东, 广州, 510310
  • Online:2005-12-20 Published:2005-12-20

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摘要： 目的 探讨HelenaSAS-3型全自动琼脂糖凝胶电泳分析系统在地中海贫血诊断中的临床应用。方法 采用琼脂糖凝胶电泳检测200份标本中的血红蛋白A和A2含量。选择两份血红蛋白液进行批内、批间精密度试验。结果 200例标本中,170例健康体检者、12例急性淋巴细胞白血病患者、12例急性髓系细胞白血病患者、4例非何杰金氏淋巴瘤患者血样血红蛋白A、血红蛋白A2的平均值±标准差分别是(%):97.55±0.51、2.44±0.51,97.01±0.329、2.99±0.32,97.42±0.57、2.84±0.28,97.44±0.55、2.55±0.55。2例β-地中海贫血患者血红蛋白电泳血红蛋白F均高于正常成人水平;血红蛋白A、A2批内、批间精密度试验CV%结果分别是:0.17、7.76,0.16、7.14。结论 Helena公司SAS-3型全自动琼脂糖凝胶电泳分析系统够清晰分辨血红蛋白A2和血红蛋白F增高者,为临床诊断地中海贫血及异常血红蛋白病提供准确的实验室方法和依据。

Abstract: Objective To assess the value of Halena SAS-3 full automatic hemoglobin agarose electrophoresis in the diagnosis of thalassemia. Methods Hemoglobin A and A2 was detected by agarose electrophoresis in 200 blood samples collected from 170 healthy subjects, 2 patients with beta-thalassemia, 12 with acute lymphoblastic leukemia, 12 acute myelocytic leukemia and with 4 non-Hodgkin lymphoma. Results The Mean±SD of hemoglobin A in the healthy subjects and patients with beta-thalassemia, acute lymphoblastic leukemia, acute myelocytic leukemia and non-Hodgkin lymphoma was 97.55±0.51, 97.01±0.329, 97.42±0.57, 97.44±0.55, and that of A2 was2.44±0.51, 2.99±0.32, 2.84±0.28, 2.55±0.55, respectively. The values of electrophoresed hemoglobin F of the 2 patients with beta-thalassemia were higher than those of the normal adults. The within-run coefficient of variation (CV) and between-run CV was 0.17 and 7.76 for hemoglobin A, and 0.16 and 7.14 for hemoglobin A2, respectively. Conclusions Halena SAS-3 full automatic agarose electrophoresis system possesses strong resolving power in detecting trace abnormal hemoglobin and accurately identifies unusual evaluation of hemoglobin A2 and F to facilitate the diagnosis of thalassemia.

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