南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (01): 91-.

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大鼠应激性溃疡自愈过程中环氧合酶表达的变化

徐俊;宋于刚;桑显富;鲍光欣;李旭;武钢;陈东升;   

  1. 南方医科大学南方医院急诊科； 南方医科大学南方医院消化内科； 南方医科大学南方医院急诊科 广东 广州 510515； 广东 广州 510515；
  • 出版日期:2006-01-20 发布日期:2006-01-20
  • 基金资助:
    广东省自然科学基金(010578)

Changes in cyclooxygenase gene expression during spontaneaus recovery from stress ulcer in rats

XU Jun, SONG Yu-gang, SANG Xian-fu, BAD Guang-xin, LI Xu, WU Gang, CHEN Dong-shen Department of Emergency, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China   

  1. 南方医科大学南方医院急诊科； 南方医科大学南方医院消化内科； 南方医科大学南方医院急诊科 广东 广州 510515； 广东 广州 510515；
  • Online:2006-01-20 Published:2006-01-20

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摘要： 目的研究大鼠应激性溃疡自愈过程中环氧合酶的表达;探讨环氧合酶在应激性溃疡自愈过程的作用机制。方法应用免疫组织化学和RT．PCR方法检测大鼠应激性溃疡自愈过程中环氧合酶蛋白和mRNA的表达变化。结果对照组的大鼠胃粘膜COX-2表达极低,而在溃疡自愈过程中其表达显著增加(P<0．05),并随自愈时间而减弱;COX-1在应激性溃疡自愈各组和对照组中均表达,自愈各组与对照组相比没有明显差异(P>0．05)。结论大鼠应激性溃疡自愈过程中COX-1和COX-2均有表达。参与了应激性溃疡的自愈过程,其作用可能主要与COX介导分泌的前列腺素有关。

Abstract: Objective To observe the changes in gene expression of cyclooxygenase (COX) during spontaneaus recovery from stress ulcer in rats exposed to water immersion and restraint stress (WRS). Methods A rat model of stress ulcer was established by means of WRS, in which the changes in COX expression were detected with immunohistochemistry and reverse transcription (RT)-PCR. Results Very low levels of COX-2 expression were detected in the gastric mucosa of the control rats, and the expression increased significantly during the healing process of the stress ulcer (P<0.05). COX-1 expression in the gastric mucosa showed no significant difference between the control group and the stress ulcer groups during healing (P>0.05). Conclusion COX-1 and COX-2 expressions in rat gastric mucosa during the recovery from stress ulcer participate in the recovery of the damaged mucosa possibly by mediating prostaglandin secretion

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