南方医科大学学报

南方医科大学学报 ›› 2022, Vol. 42 ›› Issue (9): 1296-1302.doi: 10.12122/j.issn.1673-4254.2022.09.04

• • 上一篇    下一篇

PCGF1在直肠腺癌中高表达，沉默后可抑制直肠腺癌的增殖

曾银珍，樊 嵘   

  1. 南方医科大学南方医院麻醉科，广东 广州 510515；天津市西青医院中心实验室，天津 300380
  • 出版日期:2022-09-20 发布日期:2022-09-28

PCGF1 is highly expressed in rectal adenocarcinoma and silencing PCGF1 inhibits proliferation of rectal adenocarcinoma cells in vitro

ZENG Yinzhen, FAN Rong   

  1. Department of Anesthesiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; Central Laboratory, Tianjin Xiqing Hospital, Tianjin 300380, China
  • Online:2022-09-20 Published:2022-09-28

RichHTML

64

PDF

439

摘要： 目的 探讨PCGF1在直肠腺癌中的表达及增殖的调控作用。方法 采用UALCAN和ENCORI数据库在线分析PCGF1在直肠腺癌组织和正常组织中的表达丰度，以及与直肠腺癌临床病理参数以及生存预后的相关性。通过qPCR和Western blot方法检测直肠腺癌细胞株和正常细胞株PCGF1表达水平。采用慢病毒载体分别构建PCGF1过表达和沉默稳定转染细胞系，将细胞分为未转染、过表达和沉默组，通过CCK-8法检测细胞增殖能力。裸鼠成瘤实验检测PCGF1对体内肿瘤增殖的影响。结果PCGF1在直肠腺癌组织中呈现高表达（P<0.001），PCGF1表达与直肠腺癌分期、组织分化及淋巴结是否转移有关（P<0.001），且PCGF1高表达的直肠腺癌患者预后更差（P<0.05）。SW837和SW1463细胞中PCGF1沉默组细胞增殖能力降低（P<0.05），且PCGF1沉默明显抑制直肠癌细胞在裸鼠体内的增殖（P<0.01）。结论 PCGF1在直肠腺癌组织中高表达，可能是直肠腺癌患者生存及预后的标志分子和潜在治疗靶标。

关键词: 多梳家族；PCGF1；直肠腺癌；增殖；生存预后

Abstract: Objective To investigate the expression of PCGF1 in rectal adenocarcinoma (READ) and the effect of PCGF1 silencing on proliferation READ cells in vitro. Methods The UALCAN and ENCORI online databases were used to analyze the expression level of PCGF1 in READ tissues and normal tissues and its association with the clinicopathological parameters and survival outcomes of patients with READ. The expression levels of PCGF1 were detected in two READ cell lines and a normal rectal epithelial cell line (HcoEpiC cells) using qPCR and Western blotting. Lentiviral vectors were used to construct PCGF1-overexpressing and PCGF1-silenced cell lines, and the proliferative activity of the cells was assessed using CCK-8 assay. The effect of PCGF1 silencing on tumor proliferation in vivo was also evaluated by observing tumorigenicity of the cells in nude mice. Results PCGF1 was highly expressed in READ tissue (P<0.001), and its expression levels was correlated with READ stage, differentiation and lymph node metastasis (P<0.001). A high PCGF1 expression level was associated with a poor survivalo utcome of READ patients (P<0.05). In SW837 and SW1463 cells, PCGF1 silencing significantly lowered the proliferative activity of the cells both in vitro (P<0.05) and in nude mice (P<0.01). Conclusion PCGF1 is highly expressed in READ tissue and may potentially serve as a prognostic biomarker as well as a therapeutic target for READ.

Key words: polycomb group; PCGF1; rectal adenocarcinoma; proliferation; survival outcomes