Abstract: Objective To investigate the role of ROG, GATA3 and T-bet in the progression of chronic hepatitis B (CHB). Methods
The mRNA levels of ROG, GATA3 and T-bet in peripheral blood mononuclear cells (PBMCs) from 135 patients with CHB
(including 45 mild cases, 42 moderate cases, and 48 severe cases) and 15 healthy control subjects were detected by real-time
quantitative PCR. Results The levels of T-bet mRNA in the PBMCs were significantly higher in CHB patients than in the
healthy controls (P<0.05), and also differed significantly between the 3 groups of CHB patients (P<0.05). ROG mRNA levels
were significantly higher in severe cases of CHB than in the healthy controls and mild and moderate CHB cases (P<0.05), but
were similar among the latter 3 groups (P>0.05). The mRNA level of GATA3 in the PBMCs were significantly higher in
moderate and severe CHB cases than in the healthy controls and mild CHB cases (P<0.05). The T-bet/GATA3 ratio was
significantly greater in the 3 CHB groups than in the control group (P<0.05) but comparable between the 3 CHB groups (P>
0.05). ROG levels were not correlated with GATA3 levels or T-bet/GATA3 ratio in the CHB cases. Conclusions The mRNA
levels of ROG, GATA3 and T-bet in the PBMCs are obviously up-regulated in CHB patients and these 3 genes may participate
in the progression of CHB. ROG plays an important role in correcting and maintaining the new balance of Th1/Th2.