Abstract: Objective To study the metabolic changes of pancreatic extracts from insulin-resistant rats induced by fructose
feeding using nuclear magnetic resonance 1H spectroscopy (1H NMR). Methods Sixteen Wistar rats were divided equally into
control group and model group and given water and 10% fructose water for 8 weeks, respectively. The pancreatic tissues were
then obtained for 1H NMR spectra analysis and principal component analysis (PCA). Results Compared with the control rats,
the rats in the model group showed significantly increased creatine, betaine/TMAO, taurine, glycine and myo-inositol and
decreased levels of lipids, lactate, glutamate, choline and GPC/PC. Conclusion 1H NMR and pattern recognition can define the
metabolic characteristics of the pancreatic tissue extracts from insulin-resistant rats and provide reliable metabolic evidence for
studying the mechanisms of insulin resistance at the molecular level.