Abstract: Objective To explore the effects of salidroside (sal) on the expressions of Bcl-2, Bax and caspases-3 proteins in
cultured rat subventricular zone (SVZ) neural stem cells (NSCs) exposed to hypoxia injury. Methods Primarily cultured SVZ
NSCs from adult SD rats were incubated with salidroside (120 and 240 μmol /L) for 24 h prior to exposure to hypoxia. The cell
viability was assessed with MTT assay, and the cell apoptosis was analyzed using TUNEL staining and flow cytometry. Western
blotting was performed to detect the expressions of Bcl-2, Bax and caspase-3 in the cells. Results Salidroside pretreatment of the
cells for 24 h resulted in an obvious resistance to hypoxia-induced cell apoptosis and decrement of cell viability (P<0.05).
Salidroside also antagonized the effect of hypoxia exposure in lowering Bcl-2/Bax ratio apoptosis of rat neural stem cells and
decreased the expression of caspases-3 protein (P<0.05). Conclusion Salidroside can significantly resist hypoxia-induced. The
neuroprotective effect of salidroside may be related to the modulation of expressions of apoptosis-related proteins.