Abstract: Objective To screen DENV-2 binding proteins from Aedes albopictus and Culex. quinquefasciatus. Methods The total
proteins of Aedes albopictus and Culex. quinquefasciatus in different developmental stages were prepared and analyzed with
SDS-12% polyacrylamide gel. After electophoresis the proteins were transferred using Mini Trans-Blot Electrophoretic
Transfer Cell (Bio-Rad ) to a nitrocellulose membrane. Virus overlay protein-binding assay (VOPBA) was carried out using
anti-dengue virus 1-4 monoclonal antibody. Results In Aedes albopictus, VOPBA detected DEN-2 binding molecules of 25 000,
35 000, and 50 000 in larvae samples, molecules of 35 000 and 50 000 in pupae samples, a 50 000 molecule in male mosquito
samples, and molecules of 35 000 and 50 000 in female mosquito samples. DENV-2 binding protein of 35 000 was found in the
larvae, pupae, and female mosquitoes, but not in male mosquitoes. In Culex. Quinquefasciatus, VOPBA detected a molecule of
100 000 in larvae samples, molecules of 40 000, 100 000, and around 50 000 (48 000 and 60 000) in pupae samples, and
molecules of 40 000 and 100 000 in male mosquitoes and female mosquito samples. Conclusion Several proteins capable of
binding DENV are found in Aedes albopictus and Culex. quinquefasciatus in different development stages. The 35 000 molecule
expressed in Aedes albopictus as a putative receptor protein may be related to virus tropism in mosquito tissues.