Bone defect repair with a new tissue-engineered bone carrying bone morphogenetic protein in rabbits

Abstract

Abstract: Objective To construct a new tissue-engineered bone with poly (D, L-lactide-co-glycolide) (PLGA), bone morphogenetic protein (BMP) and bone marrow-derived stem cells (BMSCs) and observe its effect in repairing segmental bone defects. Methods A 15-mm bone defect in the right radius was induced in New Zealand white rabbits, and the models were randomized into three groups to receive implantation of the tissue-engineered bone grafts constructed with PLGA carrying 5 mg BMP and about 1×10⁶ BMSCs (experimental group), grafts of PLGA with about 1×10⁶ BMSCs (control group), or grafts of exclusive PLGA (blank control group), respectively. The osteogenesis in the bone defect after the implantation on was evaluated X-ray films, and the histological changes of the tissues sampled from the bone defect 4, 8, and 12 weeks after operation were observed and new bone formation was measured by image analysis. Results The bone defect was completely repaired in the experimental group 12 weeks after the implantation, showing the best results among the 3 groups. The bone defects in the blank control group was filled with only fibrous and connective tissues at 12 weeks. Conclusion This tissue-engineered bone constructed with PLGA, BMP and BMSCs possesses good ability in repairing segmental bone defect.

CLC Number:

R322.71

HU Ji-jie¹, JIN dan¹, QUAN Da-ping², ZHONG Shi-zhen³, CHEN Jiong-hao¹, WEI Kuan-hai¹, ZHAO Jie², PEI Guo-xian¹. Bone defect repair with a new tissue-engineered bone carrying bone morphogenetic protein in rabbits[J]. Journal of Southern Medical University, 2005, 25(11): 1369-1374.

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