[1]曾晓媛,焦营营,李宗鹏,等.血小板生成素通过修复化疗后骨髓内皮祖细胞促进巨核细胞造血[J].南方医科大学学报,2020,(08):1134-1140.
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血小板生成素通过修复化疗后骨髓内皮祖细胞促进巨核细胞造血()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2020年08期
页码:
1134-1140
栏目:
出版日期:
2020-07-30

文章信息/Info

Title:
Thrombopoietin promotes megakaryopoiesis via protecting bone marrow endothelial function in patients undergoing chemotherapy for hematological malignancies
作者:
曾晓媛焦营营李宗鹏张玉娇叶洁瑜
关键词:
血小板生成素骨髓内皮祖细胞巨核细胞造血
Keywords:
thrombopoietin endothelial progenitor cells megakaryopoiesis
文献标志码:
A
摘要:
目的 探索血小板生成素(TPO)能否通过修复大剂量化疗后患者的骨髓内皮祖细胞(BM-EPC)恢复其对巨核细胞的造血支持作用。方法 选取23例血液系统恶性肿瘤患者化疗后30 d的骨髓以及10例健康人骨髓作为实验标本。采用表面特异性抗原CD34,CD309,CD133对BM-EPC进行鉴定;使用CCK8分析TPO是否可促进血液肿瘤患者化疗后BM-EPC增殖及其最佳作用浓度。设置TPO处理组为实验组,无TPO为对照组,健康人BM-EPC为健康对照组;通过DiL-Ac-LDL摄取及FITC-UEA-I结合实验检测实验组、对照组和健康对照组的BM-EPC数量。利用成管及迁移实验评估3组的BM-EPC功能;分别将实验组和对照组BM-EPC与巨核细胞共培养,利用流式细胞术检测巨核细胞增殖情况。结果 实验组BM-EPC高表达CD34/CD133/CD309;TPO可促进BM-EPC增殖,最佳作用浓度为100 μg/L。免疫荧光双标实验显示,实验组BM-EPC数量较对照组明显增加(P<0.05)。实验组成管能力及迁移能力较对照组增强(P<0.05)。共培养后,实验组巨核细胞数多于对照组(P=0.013)。结论 大剂量化疗后患者BM-EPC受损,TPO具有直接刺激巨核系统造血作用,还可能通过促进BM-EPC增殖,修复其功能,从而恢复BM-EPC对巨核细胞的造血支持作用。
Abstract:
Objective To explore whether thrombopoietin (TPO) can rescue megakaryopoiesis by protecting bone marrow�2;derived endothelial progenitor cells (BM-EPCs) in patients receiving chemotherapy for hematological malignancies. Methods Bone marrow samples were collected from 23 patients with hematological malignancies 30 days after chemotherapy and from 10 healthy volunteers. BM-EPCs isolated from the samples were identified by staining for CD34, CD309 and CD133, and their proliferation in response to treatment with TPO was assessed using CCK8 assay. DiL-Ac-LDL uptake and FITC-UEA-I binding assay were performed to evaluate the amount of BM-EPCs from the subjects. Tube-formation and migration experiments were used for functional assessment of the BM-EPCs. The BM-EPCs with or without TPO treatment were co-cultured with human megakaryocytes, and the proliferation of the megakaryocytes was detected with flow cytometry. Results Flow cytometry indicated that the TPO-treated cells had high expressions of CD34, CD133, and CD309. CCK8 assay demonstrated that TPO treatment enhanced the proliferation of the BM-EPCs, and the optimal concentration of TPO was 100 μg/L. Double immunofluorescence assay indicated that the number of BM-EPC was significantly higher in TPO-treated group than in the control group. The TPO-treated BM-EPCs exhibited stronger tube-formation and migration abilities (P<0.05) and more significantly enhanced the proliferation of co-cultured human megakaryocytes than the control cells (P<0.05). Conclusion TPO can directly stimulate megakaryopoiesis and reduce hemorrhage via protecting the function of BM-EPCs in patients following chemotherapy for hematological malignancies.

相似文献/References:

[1]宁云山,李妍,周明乾,等.内含子和5’非翻译区对人血小板生成素基因表达的影响[J].南方医科大学学报,2004,(09):991.
 NING Yun-shan,LI Yan,ZHOU Ming-qian,et al.Role of intron and 5’ untranslated region in human thrombopoietin gene expression[J].Journal of Southern Medical University,2004,(08):991.

更新日期/Last Update: 2020-07-28