[1]周凌云,鲁 斌,孙 玉.东方栓孔菌多糖对小鼠的降血糖和降血脂作用[J].南方医科大学学报,2020,(08):1127-1133.
 Glucose- lowering effect of Trametes orientalis polysaccharides in hyperglycemic andhyperlipidemic mice[J].Journal of Southern Medical University,2020,(08):1127-1133.
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东方栓孔菌多糖对小鼠的降血糖和降血脂作用()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2020年08期
页码:
1127-1133
栏目:
出版日期:
2020-07-30

文章信息/Info

Title:
Glucose- lowering effect of Trametes orientalis polysaccharides in hyperglycemic and hyperlipidemic mice
作者:
周凌云鲁 斌孙 玉
关键词:
东方栓孔菌多糖降血糖降血脂
Keywords:
Trametes orientalis polysaccharides hypoglycemia hypolipidemia
文献标志码:
A
摘要:
目的 探讨东方栓孔菌多糖组分的降血糖和降血脂作用。方法 东方栓孔菌粗多糖经DEAE纤维素柱分离,利用水和0.5 mol/L NaCl溶液冲洗,收集其中多糖含量较高的组分DEAE-水和DEAE-盐,两部分分别经Sephadex G-100凝胶柱得到4个多糖组分TOPW-1,TOPW-2,TOPS-1,TOPS-2。通过2,2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)法考察各多糖组分抗氧化活性,对紫外检测信号一致的收集液TOPW-1,TOPS-1利用HPGPC测定相对分子质量,对均一的TOPW-1通过酸水解结合HPLC分析测定单糖组成。体外检测TOPW-1,TOPS-1对DPP4(二肽基肽酶-4)抑制活性,脂肪细胞摄取葡萄糖能力和对胰脂肪酶的抑制活性,初步判断降糖作用,并建立高脂饲料高血脂小鼠模型和链脲霉素(STZ)高血糖小鼠模型,实验动物分为正常组,模型组,模型+TOPS-1高、中、低剂量组(0.1,0.2,0.4 g/kg),模型+丹参(0.5 g/kg)组和模型+消渴丸(5丸/kg)组,后两组为阳性对照组,灌胃给药。采用血糖测试仪和全自动生化分析仪分别测定血清血脂指标[总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)]和血糖水平,评价TOPS-1降血糖和降血脂作用,探讨可能的作用机制。结果 TOPW-1为均一组分,是由D-甘露糖、D-葡萄糖、D-半乳糖及D-岩藻糖组成的多糖,但TOPW-1的抗氧化及体外降血糖作用均不显著。TOPS-1为非均一多糖,具有较强的抗氧化能力(在500 μg/mL浓度下,其清除率达到90.15%),对胰脂肪酶有一定的促进作用,进一步动物实验表明TOPS-1中剂量组(0.2 g/kg)能显著降低高血糖小鼠的空腹血糖值及高血脂小鼠的TC、TG和LDL-C含量,且给药过程中未出现小鼠死亡。TOPS-1低、高剂量组降血糖作用不显著,高剂量组给药存在一定增加小鼠死亡率的可能。结论 东方栓孔菌多糖具有抗氧化,降血糖和调血脂的作用,推测其降糖作用与降低氧化应激水平,减少糖尿病人脂肪代谢紊乱有关。
Abstract:
Objective To explore the glucose-lowering effect of the polysaccharide fractions of Trametes orientalis. Methods The crude polysaccharides of T. orientalis were chromatographed on DEAE cellulose column using H2O and 0.5 mol/L NaCl solution as the eluent and DEAE-water and DEAE-salt with high polysaccharide contents were collected. The two fractions were separated using Sephadex G-100 gel column to obtain 4 polysaccharide fractions TOPW-1, TOPW-2, TOPS-1, and TOPS-2. The anti-oxidation activity of the polysaccharide fractions was investigated with ABTS method. The fractions TOPW-1 and TOPS-1 with consistent UV detection signals were collected and HPGPC was used to determine their relative molecular mass. The monosaccharide composition in homogeneous TOPW-1 was determined by acid hydrolysis combined with HPLC. The in vitro inhibitory activities of TOPW-1 and TOPS-1 against DPP4, adipocyte glucose intake and lipase activity were tested to preliminarily assess their glucose-lowering effect. In a mouse model of hyperglycemic and hyperlipidemic, the glucose-lowering effect of TOPS-1 (0.1, 0.2, and 0.4 g/kg) and its effect on blood lipid metabolism were investigated in comparison with Xiaoke pills (5 pills/kg) and Danshen tablets (0.5 g/kg). Results TOPW-1 was a homogeneous polysaccharide composed mainly of D-mannose, D-glucose, D-galactose, and D-fucose, with weak antioxidant and hypoglycemic effects. TOPS-1 was not a single polysaccharide and at the concentration of 500 μg/mL showed an high ABTS clearance rate (90.15%). In the mouse model of hyperglycemia and hyperlipidemia, treatment with TOPS-1 (0.2 g/kg) enhanced the activity of lipase and significantly reduced fasting glucose level and serum contents of total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol without causing death in the mice. The glucose-lowering effects of TOPS-1 was not significant at the low (0.1 g/kg) or high (0.4 g/kg) dose, and a high dose tended to increase the mortality of the mice. Conclusion T. orientalis polysaccharides have anti-oxidation, glucose-lowering and lipid-lowering effects in mice, and their glucose-lowering effect is probably medicated by reducing oxidative stress and ameliorating lipid metabolism disorder.

相似文献/References:

[1]文晓芸,朱正光,林壁润,等.一种新的多糖——链霉菌多糖的分离与结构确证[J].南方医科大学学报,2005,(03):251.
 WEN Xiao-yun,ZHU Zheng-guang,LIN Bi-run,et al.Isolation and structural analysis of a new polysaccharid, streptomyces polysaccharide[J].Journal of Southern Medical University,2005,(08):251.

更新日期/Last Update: 2020-07-29